PMID- 12513811
OWN - NLM
STAT- MEDLINE
DCOM- 20030211
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 10
IP  - 2
DP  - 2002 Apr
TI  - [Comparison of detection of trisomy 8 with fluorescence in situ hybridization and 
      conventional karyotype analysis in myelodysplastic syndrome].
PG  - 115-8
AB  - The purpose of this study was to compare the detection of trisomy 8 in 
      myelodysplastic syndrome (MDS) patients with interphase fluorescence in situ 
      hybridization (FISH) and cytogenetic karyotype analysis. Using Spectrum Green 
      labeled chromosome 8 centromere probe, interphase FISH was established. The 
      trisomy 8 clones were simultaneously detected in 48 MDS cases with FISH and 
      conventional cytogenetic analysis (CCA). Results showed that the CCA revealed no 
      significant difference of constitutional proportion between MDS-RA and MDS-RAEB 
      with karyotypes of whole +8, partial +8 and one +8. With FISH, detectable rates 
      were 66.1% for whole +8. Partial +8 and sole +8 were significantly higher than 
      one +8 and complex +8, respectively. The percentages of trisomy 8 were similar in 
      MDS-RA and MDS-RAEB. Trisomy 8 was detected in 1 of 15 specimens with normal or 
      abnormal karyotype without trisomy 8 by FISH. There was linear correlation 
      between the percentages of partial +8 detected by FISH and CCA. Two patients 
      received CCA and FISH examination at diagnosis and during treatment, the 
      percentage of trisomy 8 was increased with progress of disease. In conclusion, 
      our results showed that FISH is a sensitive and accurate technique to detect 
      trisomy 8 in MDS patients. It can provide contribute to diagnosis, assessment of 
      curative effect and predicting progress of disease in MDS. Clone size of trisomy 
      8 does not related to classification of MDS, but sole +8 is seems to see in 
      MDS-RA frequently.
FAU - Zhang, Zhi-Biao
AU  - Zhang ZB
AD  - Henan Anyang People's Hospital, Anyang 455000, China.
FAU - Liu, Shi-He
AU  - Liu SH
FAU - Li, Juan
AU  - Li J
FAU - Bo, Li-Jin
AU  - Bo LJ
FAU - Cui, Huan-Ying
AU  - Cui HY
FAU - Liu, Xu-Ping
AU  - Liu XP
FAU - Nie, Yan-Xia
AU  - Nie YX
FAU - Qin, Shuang
AU  - Qin S
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Myelodysplastic Syndromes/*genetics/pathology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Trisomy
EDAT- 2003/01/07 04:00
MHDA- 2003/02/13 04:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2002 Apr;10(2):115-8.