PMID- 12513829
OWN - NLM
STAT- MEDLINE
DCOM- 20030203
LR  - 20171116
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 10
IP  - 1
DP  - 2002 Feb
TI  - [Expression and function of CD95/fas antigen and Bcl-2 on cord blood 
      hematopoietic progenitor cells].
PG  - 13-6
AB  - The cell-surface expression and functional status of the CD95/Fas antigen on 
      primitive hematopoietic progenitors isolated from human cord blood were studied. 
      The CD34(+) cells freshly isolated from cord blood displayed low CD95 expression, 
      combinations of cytokines such as SCF+FL upregulated the expression of CD95 in 
      CD34(+) cells. Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma 
      (IFN-gamma) further increased the CD95 expression induced by positive cytokines. 
      The functional status of CD95-mediated apoptosis was analysed by incubation of 
      CD34(+) cells in the presence of anti-CD95 monocloned antibody (McAb). The effect 
      of anti-CD95 McAb was measured by viable cells counting, flow cytometry, LTC-IC 
      and CFU-C assays. Viable cells and CFU-C numbers were 31.9 +/- 11.2 and 43 +/- 
      2.0 respectively, the rate of apoptosis was 42.9 +/- 12.4 in the presence of 
      anti-CD95 McAb and TNF-alpha or IFN-gamma. However, growth factor deprivation or 
      the early-acting cytokine such as SCF and FL cross-linking of CD95 lead to low 
      apoptosis of CD34(+) cells. The correlation of increased intracytoplasmic levels 
      of Bcl-2 and the presence of CD95 on surface of CD34(+) cells suggests that Bcl-2 
      may be involved in protecting against CD95-mediated apoptosis of cord blood 
      CD34(+) cell.
FAU - Ma, Yan-Ping
AU  - Ma YP
AD  - Department of Hematology, Xiehe Hospital, Tongji Medical College of Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Zhou, Ping
AU  - Zhou P
FAU - Xiao, Juan
AU  - Xiao J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Antibodies)
RN  - 0 (Antigens, CD34)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Antibodies/immunology
MH  - Antigens, CD34/analysis
MH  - Apoptosis
MH  - Female
MH  - Fetal Blood/*metabolism
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Humans
MH  - Proto-Oncogene Proteins c-bcl-2/*biosynthesis/physiology
MH  - fas Receptor/*biosynthesis/immunology/physiology
EDAT- 2003/01/07 04:00
MHDA- 2003/02/04 04:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/02/04 04:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2002 Feb;10(1):13-6.