PMID- 12513926
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20081121
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 15
IP  - 4
DP  - 2002
TI  - Cytokine mRNA profiles in bronchoalveolar cells of piglets experimentally 
      infected in utero with porcine reproductive and respiratory syndrome virus: 
      association of sustained expression of IFN-gamma and IL-10 after viral clearance.
PG  - 549-56
AB  - An experimental model was used to investigate mRNA cytokine profiles in 
      bronchoalvolar cells (BALC) from piglets, infected in utero with porcine 
      reproductive and respiratory syndrome virus (PRRSV). The BALC's were analyzed for 
      the cytokines TNF-alpha, IFN-gamma, IL-8, IL-10, and IL-12(p40) by real-time 
      TaqMan polymerase chain reaction in 2-, 4-, and 6-week-old piglets, respectively. 
      High levels of IFN-gamma mRNA was detected in all piglets, while IL-10 was 
      upregulated in 2-week-old piglets, was at normal levels in 4-week-old piglets, 
      and elevated again in 6-week-old piglets. IL-12 was weakly elevated in all three 
      age groups. Virus was reduced by 50% in 4-week-old piglets and cleared by 6 weeks 
      of age. The sustained expression of IFNgamma and reduction of IL-10 production 
      indicate an important role for these cytokines in immunity to PRRSV.
FAU - Johnsen, C K
AU  - Johnsen CK
AD  - Department of Immunology and Biochemistry, Copenhagen, Denmark. cjo@ssi.dk
FAU - Botner, A
AU  - Botner A
FAU - Kamstrup, S
AU  - Kamstrup S
FAU - Lind, P
AU  - Lind P
FAU - Nielsen, J
AU  - Nielsen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/*cytology
MH  - Cytokines/*metabolism
MH  - Female
MH  - Infectious Disease Transmission, Vertical
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Pregnancy
MH  - RNA, Messenger/*metabolism
MH  - Swine
EDAT- 2003/01/07 04:00
MHDA- 2003/02/14 04:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
AID - 10.1089/088282402320914494 [doi]
PST - ppublish
SO  - Viral Immunol. 2002;15(4):549-56. doi: 10.1089/088282402320914494.