PMID- 12514090
OWN - NLM
STAT- MEDLINE
DCOM- 20031112
LR  - 20061115
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 24
IP  - 1
DP  - 2003 Jan-Feb
TI  - Analysis of aneuploidy in spermatozoa from testicular biopsies from men with 
      nonobstructive azoospermia.
PG  - 100-3
AB  - Testicular sperm biopsy combined with intracytoplasmic sperm injection (ICSI) 
      allows men with azoospermia the possibility of fathering a child. However, little 
      information exists on the risk of chromosome abnormalities in their sperm. 
      Multicolor fluorescence in situ hybridization (FISH) analysis was used to 
      determine the frequency of sperm diploidy and disomy for the sex chromosomes in 
      six men with normal karyotypes and non-obstructive azoospermia. A new method 
      using microwave decondensation and codenaturation of sperm nuclei yielded a much 
      larger number of sperm nuclei for FISH analysis than our previous study of men 
      with azoospermia. A total of 59916 sperm were analyzed; more than 9000 sperm were 
      scored for each man. The men with nonobstructive azoospermia had an increased 
      frequency of sperm chromosomal disomy for YY, XY, total sex chromosomal disomy, 
      and diploidy compared with 18 normal controls, but only YY disomy reached 
      statistical significance. One infertile man had a frequency of 3.8% XY disomy and 
      4.3% diploidy, which was 13-fold and 7-fold higher than control donors, 
      respectively. Our results suggest that some men with nonobstructive azoospermia 
      have a significantly increased frequency of sex chromosomal abnormalities than 
      normal men, but that the overall frequency of abnormalities is similar to that 
      found in infertile men with abnormal semen parameters.
FAU - Martin, Renee H
AU  - Martin RH
AD  - Department of Medical Genetics, Faculty of Medicine, Alberta Children's Hospital, 
      University of Calgary, Alberta, Canada. rmartin@ucalgary.ca
FAU - Greene, Calvin
AU  - Greene C
FAU - Rademaker, Alfred W
AU  - Rademaker AW
FAU - Ko, Evelyn
AU  - Ko E
FAU - Chernos, Judy
AU  - Chernos J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Biopsy
MH  - Case-Control Studies
MH  - Diploidy
MH  - Gene Frequency
MH  - Humans
MH  - Male
MH  - Oligospermia/*genetics/pathology
MH  - Sex Chromosomes/genetics
MH  - Spermatozoa/*physiology
MH  - *Testis/pathology
MH  - Uniparental Disomy
EDAT- 2003/01/07 04:00
MHDA- 2003/11/13 05:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/11/13 05:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
PST - ppublish
SO  - J Androl. 2003 Jan-Feb;24(1):100-3.