PMID- 12514394
OWN - NLM
STAT- MEDLINE
DCOM- 20030123
LR  - 20151119
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 113
IP  - 1
DP  - 2003 Jan
TI  - The pathogenesis of nasal polyposis by immunoglobulin E and interleukin-5 is 
      completed by transforming growth factor-beta1.
PG  - 120-4
AB  - OBJECTIVES/HYPOTHESIS: Nasal polyps are benign mucosal protrusions into the nasal 
      cavity of multifactorial origin and are characterized by chronic mucosal 
      inflammation. The suggested multifactorial pathological mechanisms comprise 
      several factors including cytokines and immunoglobulin E (IgE). The study was 
      designed to examine the suggested roles of IgE, interleukin-5 (IL-5), and 
      transforming growth factor-beta1 (TGF-beta1) in the pathogenesis of nasal 
      polyposis. METHODS: Nasal polyps (n = 34) and healthy nasal mucosa samples (n = 
      9) were taken during routine endonasal surgeries. Immunoglobulin E (n = 13), IL-5 
      (n = 22), and TGF-beta1 (n = 27) concentrations were measured with enzyme-linked 
      immunosorbent assay technique in homogenized polyp tissue and in control mucosa. 
      Atopic and nonatopic groups were selected and compared. Histomorphological 
      examination and immunohistochemical analysis to detect IL-5 and TGF-beta1 were 
      performed in five specimens. RESULTS: The level of tissue-bound IgE was 
      significantly higher in polyps compared with control specimens and in atopic 
      compared with nonatopic polyps, but between nonatopic polyps and control 
      specimens the difference was not significant. However, significant correlation 
      was found between tissue and serum IgE in the complete polyp (P =.001) and atopic 
      polyps group (P =.05). Tissue IL-5 concentration was significantly higher in 
      polyps compared with control specimens, in which it was below the limit (15 
      pg/mL), and there was no difference between atopic and nonatopic polyps. In 
      atopic polyps there was significant correlation between tissue IgE and IL-5. 
      Transforming growth factor-beta1 concentration proved to be significantly higher 
      in control mucosa than in polyps, with no difference between atopic and nonatopic 
      polyps. Immunohistochemical analysis revealed numerous IL-5-positive eosinophil 
      cells and TGF-beta1 positivity in the lamina propria of polyp samples, but none 
      in control specimens. CONCLUSIONS: High tissue TGF-beta1 quantity in healthy 
      nasal mucosa without its active form on the cell surface and its low quantity in 
      polyps may reflect its essential role in the inhibitory mechanisms of nasal 
      polyposis. Interleukin-5 plays a key role in the eosinophil recruitment and 
      activation, and both atopic and nonatopic pathways might activate this process. 
      The main sources of IL-5 and TGF-beta1 are the eosinophils and macrophages. 
      Immediate hypersensitivity, besides other mechanisms, might be related to atopic 
      polyps, but the involvement of other, local allergic mechanisms in IgE production 
      of nonatopic polyp tissue cannot be excluded.
FAU - Hirschberg, Andor
AU  - Hirschberg A
AD  - Department of Otorhinolaryngology--Head and Neck Surgery, Faculty of Medicine, 
      Semmelweis University, Budapest, Hungary. hirschberg@fulo.sote.hu
FAU - Jokuti, Adrienn
AU  - Jokuti A
FAU - Darvas, Zsuzsa
AU  - Darvas Z
FAU - Almay, Krisztina
AU  - Almay K
FAU - Repassy, Gabor
AU  - Repassy G
FAU - Falus, Andras
AU  - Falus A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Biomarkers)
RN  - 0 (Interleukin-5)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers/analysis
MH  - Biopsy, Needle
MH  - Case-Control Studies
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/analysis/*metabolism
MH  - Immunohistochemistry
MH  - Interleukin-5/analysis/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Nasal Mucosa/*metabolism/pathology
MH  - Nasal Polyps/immunology/*pathology/surgery
MH  - Probability
MH  - Prognosis
MH  - Sampling Studies
MH  - Sensitivity and Specificity
MH  - Statistics, Nonparametric
MH  - Transforming Growth Factor beta/analysis/*metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2003/01/07 04:00
MHDA- 2003/01/24 04:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/01/24 04:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
AID - 10.1097/00005537-200301000-00022 [doi]
PST - ppublish
SO  - Laryngoscope. 2003 Jan;113(1):120-4. doi: 10.1097/00005537-200301000-00022.