PMID- 12514431
OWN - NLM
STAT- MEDLINE
DCOM- 20030626
LR  - 20191106
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 26
IP  - 1
DP  - 2003 Jan-Feb
TI  - Distinctive maturation of in vitro versus in vivo anti-CD40 mAb-matured dendritic 
      cells in mice.
PG  - 72-84
AB  - Dendritic cells (DCs) can be matured by CD40 stimulation to upregulate their MHC 
      class II/peptide complexes and costimulatory molecule surface expression to 
      become adept at presenting antigen to and activating naive T lymphocytes. The use 
      of anti-CD40 antibodies as adjuvants for DC-based therapy has been advanced. 
      Little is known as to how DC biology in response to CD40 ligation differs between 
      in vitro versus in vivo ligation. Therefore, the authors analyzed the expression 
      kinetics of MHC class II (I-Ak)/HEL peptide "complex," total MHC class II, CD80, 
      and CD86 on in vitro or in vivo CD40-stimulated DCs over a period of 5 days. MHC 
      class II, "complex," and costimulatory molecule expression was elevated at 1 day 
      in vitro and stayed high for the culture period, whereas in vivo expression of 
      the cohort of molecules peaked earlier and then declined. When purified DCs were 
      co-cultured in vitro with antigen-specific T cell hybridomas, the DCs had lower 
      expression of total MHC class II and "complex," but did not reduce their CD80 and 
      CD86 expression. The lower expression was dependent on cognate interaction as a 
      non-antigen-specific T cell hybridoma was without effect. Blocking 
      antigen-specific MHC class II/peptide-T cell receptor (TcR) complex interaction 
      with antibody inhibited the reduction of MHC class II expression on 
      CD40-stimulated DCs in vitro. Overall, their studies suggest distinct response of 
      DCs to typical conditions that feature anti-CD40 monoclonal antibody 
      (mAb)-activated DCs in vitro or in vivo.
FAU - Frleta, Davor
AU  - Frleta D
AD  - Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, New 
      Hampshire 03756, U.S.A.
FAU - Lin, Jack T
AU  - Lin JT
FAU - Quezada, Sergio A
AU  - Quezada SA
FAU - Wade, Terri K
AU  - Wade TK
FAU - Barth, Richard J
AU  - Barth RJ
FAU - Noelle, Randolph J
AU  - Noelle RJ
FAU - Wade, William F
AU  - Wade WF
LA  - eng
GR  - CA076612/CA/NCI NIH HHS/United States
GR  - P30 CA23108/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD40 Antigens)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antigen Presentation
MH  - CD40 Antigens/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Genes, MHC Class II/*genetics
MH  - In Vitro Techniques
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Models, Animal
MH  - Probability
MH  - Up-Regulation
EDAT- 2003/01/07 04:00
MHDA- 2003/06/27 05:00
CRDT- 2003/01/07 04:00
PHST- 2003/01/07 04:00 [pubmed]
PHST- 2003/06/27 05:00 [medline]
PHST- 2003/01/07 04:00 [entrez]
AID - 10.1097/00002371-200301000-00008 [doi]
PST - ppublish
SO  - J Immunother. 2003 Jan-Feb;26(1):72-84. doi: 10.1097/00002371-200301000-00008.