PMID- 12516428
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20061115
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 14
IP  - 6
DP  - 2000 Nov
TI  - [Neurobiological effect of nerve regeneration conditioned fluid on motoneuron].
PG  - 325-7
AB  - OBJECTIVE: To explore the mechanism of tissue specificity of neurotropism in 
      peripheral nerve regeneration, we investigated the biological characteristics of 
      the nerve regeneration conditioned fluids(NRCF) on motoneuron of SD rats cultured 
      in vitro. METHODS: Silicon chambers were sutured respectively to the distal 
      stumps of motorial branch of femoral nerve and saphenous nerve to collect NRCF, 
      namely MD-NRCF and SD-NRCF. The rats cortex motoneuron were divided into 4 groups 
      and cocultured with MD-NRCF, SD-NRCF, b-FGF and serum-free medium respectively. 
      The cultured cells were photoed under phase-contrast microscope, their longest 
      neurites and cell-body areas were measured by cell image processing computer 
      system. MTT automated colorimetric microassay was also adopted to quantify the 
      activation of cultured motoneurons in each group. RESULTS: Cells of MD-NRCF group 
      had longer neurites than those of the other three groups, and their activation 
      was also superior to those of the other groups. CONCLUSION: The results suggest 
      that MD-NRCF has more significantly neurite-promoting and neurobiological effects 
      on motoneuron than SD-NRCF and b-FGF.
FAU - Ping, P
AU  - Ping P
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011.
FAU - Li, Q F
AU  - Li QF
FAU - Gan, J L
AU  - Gan JL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Nerve Growth Factors)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Female
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Male
MH  - Motor Neurons/*cytology/drug effects
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Regeneration/*drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/01/09 04:00
MHDA- 2003/02/07 04:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2000 Nov;14(6):325-7.