PMID- 12516629
OWN - NLM
STAT- MEDLINE
DCOM- 20030416
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 48
IP  - 4
DP  - 2002 Oct
TI  - IL-18 is present at the maternal-fetal interface and enhances cytotoxic activity 
      of decidual lymphocytes.
PG  - 191-200
AB  - PROBLEM: Perforin expressing uterine natural killer (uNK) cells are under complex 
      cytokine influence. The aim of the study was to investigate the presence and role 
      of interleukin (IL)-18 on NK cytolytic potential at maternal-fetal (M-F) 
      interface. METHOD OF STUDY: Peripheral blood cells and decidual tissue were 
      obtained from elective pregnancy termination of normal human 6-10-week-old 
      pregnancies. Perforin expression and cytolytic activity of peripheral blood (PBL) 
      and decidual lymphocytes (DL) were analyzed by flow cytometry. IL-18 positive 
      decidual adherent cells (DAC) were detected by the same method. Interleukin-18 
      and IL-18 receptor (IL-18R) expression on the trophoblastic cells was detected by 
      immunohistology using biotinylated anti-IL-18 and IL-18R monoclonal antibodies. 
      RESULTS: The IL-18 added in a dose of 10 ng/mL up-regulates perforin expression 
      and cytolytic activity of DL. Simultaneous stimulation with IL-18 and IL-12 
      enhanced DL cytolytic activity, while IL-18 combined with IL-10 or IL-15 did not 
      show this effect. Cytolytic activity of PBL was up-regulated by IL-18 as well, 
      and this effect was enhanced by the addition of IL-12 and IL-15. Interleukin-18 
      did not affect perforin-protein expression in cultured PBL. Approximately 20% of 
      DAC were IL-18 positive and these cells were mostly human leukocyte antigen 
      (HLA)-DR negative. IL-18R positive cells were found on syncytiotrophoblast cell 
      layer, interstitial tissue cells of villi and fetal blood cells. There was no 
      detectable IL-18 staining on trophoblast cell layer on villi, but strong staining 
      of fetal blood cells in villous vessels. CONCLUSION: These are first results 
      showing IL-18R expression, but not IL-18 expression on villous trophoblastic 
      cells, as well as enhancement of perforin expression and NK cytolytic potential 
      of DL under the influence of IL-18. IL-18 in concert with other cytokines and 
      hormones could play an important role in the regulation of cytolytic potential of 
      first trimester pregnancy decidual and peripheral blood NK cells.
FAU - Tokmadzic, Vlatka S
AU  - Tokmadzic VS
AD  - Department of Physiology and Immunology, Medical Faculty, University of Rijeka, 
      Rijeka, Croatia.
FAU - Tsuji, Yoshiyuki
AU  - Tsuji Y
FAU - Bogovic, Tatjana
AU  - Bogovic T
FAU - Laskarin, Gordana
AU  - Laskarin G
FAU - Cupurdija, Kristijan
AU  - Cupurdija K
FAU - Strbo, Natasa
AU  - Strbo N
FAU - Koyama, Koji
AU  - Koyama K
FAU - Okamura, Haruki
AU  - Okamura H
FAU - Podack, Eckhard R
AU  - Podack ER
FAU - Rukavina, Daniel
AU  - Rukavina D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 126465-35-8 (Perforin)
SB  - IM
MH  - Adult
MH  - Chorionic Villi/metabolism
MH  - Decidua/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-18/*metabolism
MH  - Interleukins/metabolism
MH  - Killer Cells, Natural/*metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins
MH  - Pregnancy
EDAT- 2003/01/09 04:00
MHDA- 2003/04/17 05:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/04/17 05:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
AID - 10.1034/j.1600-0897.2002.01132.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2002 Oct;48(4):191-200. doi: 
      10.1034/j.1600-0897.2002.01132.x.