PMID- 12517057
OWN - NLM
STAT- MEDLINE
DCOM- 20030128
LR  - 20161013
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 101
IP  - 11
DP  - 2002 Nov
TI  - Feasibility of human T-lymphotropic virus type I screening using pooled sera.
PG  - 775-8
AB  - BACKGROUND AND PURPOSE: The human T-cell lymphotropic virus type I (HTLV-I) 
      seroprevalence rate among volunteer blood donors in Taiwan is low. To study the 
      feasibility of HTLV-I enzyme immunoassay (EIA) screening using pooled sera, we 
      prospectively compared its sensitivity to that of the routine test for each 
      donor. METHODS: HTLV-I EIA tests for each serum sample and a pooled-sera test 
      with 50 samples from voluntary donated blood samples were performed concurrently 
      to assess the effectiveness and cost savings of this screening method. RESULTS: 
      Of 135,606 blood samples from volunteer donors tested for HTLV-I infection, 60 
      samples (0.044%) were HTLV-I EIA-positive using the routine method. Among these, 
      the positive results were confirmed by Western blot in 22 samples (36.7%). In the 
      pooled-sera test, 17 of 2,713 pooled samples were EIA-positive and these results 
      were all confirmed by Western blot. Five of the 22 (22.7%) EIA-positive samples 
      had a false-negative result in the pooled-sera test. Serial dilution in these 
      five cases revealed that the maximum dilution before loss of sensitivity was 
      8-fold for two specimens and 16-fold for three specimens. CONCLUSION: In this 
      study, the 50-pooled sera test had higher specificity (100%), but lower 
      sensitivity (77.3%), than the routine HTLV-I screening. Our results suggest that 
      use of a pooling method with five samples would leave a reasonable safety margin 
      and be feasible for HTLV-I mass screening in areas with low seroprevalence for 
      HTLV-I infection.
FAU - Chang, Chao-Sung
AU  - Chang CS
AD  - Blood Bank, Kaohsiung Municipal Hsiao-kang Hospital, Kaohsiung, Taiwan.
FAU - Wu, Ya-Wen
AU  - Wu YW
FAU - Pan, Ya-Chun
AU  - Pan YC
FAU - Chen, Zi-Yue
AU  - Chen ZY
FAU - Wang, Ching-Shan
AU  - Wang CS
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
RN  - 0 (HTLV-I Antibodies)
SB  - IM
MH  - Blood Specimen Collection/*methods
MH  - Blotting, Western
MH  - Feasibility Studies
MH  - HTLV-I Antibodies/isolation & purification
MH  - HTLV-I Infections/*epidemiology
MH  - Human T-lymphotropic virus 1/*isolation & purification
MH  - Humans
MH  - Mass Screening/*methods
MH  - Sensitivity and Specificity
MH  - Seroepidemiologic Studies
MH  - Taiwan/epidemiology
EDAT- 2003/01/09 04:00
MHDA- 2003/01/29 04:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/01/29 04:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
PST - ppublish
SO  - J Formos Med Assoc. 2002 Nov;101(11):775-8.