PMID- 12517381
OWN - NLM
STAT- MEDLINE
DCOM- 20030513
LR  - 20190822
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 59
IP  - 3-4
DP  - 2003 Feb
TI  - Effects of pregnant mare serum gonadotropin (eCG) on follicle development and 
      granulosa-cell apoptosis in the pig.
PG  - 775-85
AB  - The effect of eCG on follicular development and granulosa-cell apoptosis in 
      sexually mature and immature gilts and on granulosa-cell apoptosis in vitro were 
      studied. The sexually mature gilts were treated with eCG on Day 11 of the estrous 
      cycle, and effects were analyzed at different times after treatment with 
      untreated animals at corresponding stages of the cycle as controls. Apoptosis was 
      determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL), hematoxylin and eosin staining, and DNA ladder. The proportion 
      of apoptotic cells in atretic follicles (39%) was significantly higher (P<0.01) 
      than that in healthy follicles (9%). At 24h after eCG treatment in mature gilts, 
      the total number of follicles visible on the ovarian surface (57 per ovary), the 
      number of small (<3mm) follicles (31.5 per ovary) and the number of medium-sized 
      (3-5mm) follicles (23 per ovary) were significantly higher (P<0.05) than those of 
      control animals (28, 20 and 6.5 per ovary, respectively), and declined gradually 
      thereafter to below the level of control animals. The number of large (>or=5mm) 
      follicles began to show a marked increase at 72h after eCG (8.5 versus 2.5, 
      P<0.05). At 24h after eCG treatment, the proportions of apoptotic cells in small 
      (7.2%) and medium-sized follicles (7.4%) were markedly lower (P<0.01) than those 
      in controls (21.5 and 21%, respectively) and increased gradually thereafter to 
      approach the level in controls. The percentage of apoptotic cells in large 
      follicles (10% at 24h post-eCG) did not change significantly. Before eCG 
      treatment, there were markedly fewer follicles of all types on ovaries of 
      immature gilts than of mature gilts (9 versus 25 per ovary) and the proportion of 
      apoptotic cells in small and medium follicles was high (25 and 34%, 
      respectively). After eCG treatment, the changes in follicle number and proportion 
      of apoptotic cells in the immature gilts followed a similar pattern to that of 
      the mature gilts. Equine chorion gonadotropin inhibited apoptosis of granulosa 
      cells cultured either in vitro or in intact follicles in a dose-dependent manner. 
      Thus, follicular atresia in the pig, as in other animals, was characterized by 
      apoptosis of large numbers of granulosa cells, and eCG promoted follicular 
      development by inhibition of granulosa-cell apoptosis.
FAU - Liu, Z H
AU  - Liu ZH
AD  - College of Life Science, Northeast Agricultural University, Harbin 150030, PR 
      China.
FAU - Yue, K Z
AU  - Yue KZ
FAU - Ma, S F
AU  - Ma SF
FAU - Sun, X S
AU  - Sun XS
FAU - Tan, J H
AU  - Tan JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Gonadotropins, Equine)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - DNA Fragmentation
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Follicular Atresia/*drug effects/physiology
MH  - Gonadotropins, Equine/*pharmacology
MH  - Granulosa Cells/*drug effects/physiology
MH  - In Situ Nick-End Labeling/veterinary
MH  - Ovarian Follicle/*drug effects/physiology
MH  - Swine/*physiology
EDAT- 2003/01/09 04:00
MHDA- 2003/05/14 05:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/05/14 05:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
AID - S0093691X02011226 [pii]
AID - 10.1016/s0093-691x(02)01122-6 [doi]
PST - ppublish
SO  - Theriogenology. 2003 Feb;59(3-4):775-85. doi: 10.1016/s0093-691x(02)01122-6.