PMID- 12517812
OWN - NLM
STAT- MEDLINE
DCOM- 20030521
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 101
IP  - 7
DP  - 2003 Apr 1
TI  - Functional and comparative analysis of globin loci in pufferfish and humans.
PG  - 2842-9
AB  - To further our understanding of the regulation of vertebrate globin loci, we have 
      isolated cosmids containing alpha- and beta-globin genes from the pufferfish Fugu 
      rubripes. By DNA fluorescence in situ hybridization (FISH) analysis, we show that 
      Fugu contains 2 distinct hemoglobin loci situated on separate chromosomes. One 
      locus contains only alpha-globin genes (alpha-locus), whereas the other also 
      contains a beta-globin gene (alpha beta-locus). This is the first poikilothermic 
      species analyzed in which the physical linkage of the alpha- and beta-globin 
      genes has been uncoupled, supporting a model in which the separation of the 
      alpha- and beta-globin loci has occurred through duplication of a locus 
      containing both types of genes. Surveys for transcription factor binding sites 
      and DNaseI hypersensitive site mapping of the Fugu alpha beta-locus suggest that 
      a strong distal locus control region regulating the activity of the globin genes, 
      as found in mammalian beta-globin clusters, may not be present in the Fugu alpha 
      beta-locus. Searching the human and mouse genome databases with the genes 
      surrounding the pufferfish hemoglobin loci reveals that homologues of some of 
      these genes are proximal to cytoglobin, a recently described novel member of the 
      globin family. This provides evidence that duplication of the globin loci has 
      occurred several times during evolution, resulting in the 5 human globin loci 
      known to date, each encoding proteins with specific functions in specific cell 
      types.
FAU - Gillemans, Nynke
AU  - Gillemans N
AD  - MGC Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
FAU - McMorrow, Tara
AU  - McMorrow T
FAU - Tewari, Rita
AU  - Tewari R
FAU - Wai, Albert W K
AU  - Wai AW
FAU - Burgtorf, Carola
AU  - Burgtorf C
FAU - Drabek, Dubravka
AU  - Drabek D
FAU - Ventress, Nicki
AU  - Ventress N
FAU - Langeveld, An
AU  - Langeveld A
FAU - Higgs, Douglas
AU  - Higgs D
FAU - Tan-Un, Kian
AU  - Tan-Un K
FAU - Grosveld, Frank
AU  - Grosveld F
FAU - Philipsen, Sjaak
AU  - Philipsen S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20021127
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Animals
MH  - Consensus Sequence
MH  - Erythrocytes
MH  - *Evolution, Molecular
MH  - Gene Duplication
MH  - Gene Expression Regulation
MH  - Globins/biosynthesis/*genetics
MH  - Humans
MH  - Locus Control Region/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Multigene Family/*genetics
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Takifugu/*genetics
EDAT- 2003/01/09 04:00
MHDA- 2003/05/22 05:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/05/22 05:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
AID - S0006-4971(20)51179-8 [pii]
AID - 10.1182/blood-2002-09-2850 [doi]
PST - ppublish
SO  - Blood. 2003 Apr 1;101(7):2842-9. doi: 10.1182/blood-2002-09-2850. Epub 2002 Nov 
      27.