PMID- 12517949
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20220129
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 170
IP  - 2
DP  - 2003 Jan 15
TI  - A critical role for Syk protein tyrosine kinase in Fc receptor-mediated antigen 
      presentation and induction of dendritic cell maturation.
PG  - 846-52
AB  - Dendritic cells (DCs) are the only APCs capable of initiating adaptive immune 
      responses. The initiation of immune responses requires that DCs 1) internalize 
      and present Ags; and 2) undergo a differentiation process, called "maturation", 
      which transforms DCs into efficient APCs. DC maturation may be initiated by the 
      engagement of different surface receptors, including certain cytokine receptors 
      (such as TNFR), Toll-like receptors, CD40, and FcRs. The early activation events 
      that link receptor engagement and DC maturation are not well characterized. We 
      found that FcR engagement by immune complexes induced the phosphorylation of Syk, 
      a protein tyrosine kinase acting immediately downstream of FcRs. Syk was 
      dispensable for DC differentiation in vitro and in vivo, but was strictly 
      required for immune complexes internalization and subsequent Ag presentation to T 
      lymphocytes. Importantly, Syk was also required for the induction of DC 
      maturation and IL-12 production after FcR engagement, but not after engagement of 
      other surface receptors, such as TNFR or Toll-like receptors. Therefore, protein 
      tyrosine phosphorylation by Syk represents a novel pathway for the induction of 
      DC maturation.
FAU - Sedlik, Christine
AU  - Sedlik C
AD  - Institut National de la Sante et de la Recherche Medicale Unite 520, Institut 
      Curie, Paris, France.
FAU - Orbach, Daniel
AU  - Orbach D
FAU - Veron, Philippe
AU  - Veron P
FAU - Schweighoffer, Edina
AU  - Schweighoffer E
FAU - Colucci, Francesco
AU  - Colucci F
FAU - Gamberale, Romina
AU  - Gamberale R
FAU - Ioan-Facsinay, Andrea
AU  - Ioan-Facsinay A
FAU - Verbeek, Sjef
AU  - Verbeek S
FAU - Ricciardi-Castagnoli, Paola
AU  - Ricciardi-Castagnoli P
FAU - Bonnerot, Christian
AU  - Bonnerot C
FAU - Tybulewicz, Victor L J
AU  - Tybulewicz VL
FAU - Di Santo, James
AU  - Di Santo J
FAU - Amigorena, Sebastian
AU  - Amigorena S
LA  - eng
GR  - FS/12/4/29254/BHF_/British Heart Foundation/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Il2rg protein, mouse)
RN  - 0 (Interleukin Receptor Common gamma Subunit)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Interleukin-7)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Bone Marrow Cells/enzymology/immunology/metabolism
MH  - Cell Differentiation/genetics/*immunology
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*cytology/*enzymology/immunology/metabolism
MH  - Enzyme Precursors/deficiency/genetics/metabolism/*physiology
MH  - Fetus
MH  - Interleukin Receptor Common gamma Subunit
MH  - Intracellular Signaling Peptides and Proteins
MH  - Liver Transplantation/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/deficiency/genetics/metabolism/*physiology
MH  - Receptors, Fc/*physiology
MH  - Receptors, IgG/physiology
MH  - Receptors, Interleukin-7/deficiency/genetics
MH  - Signal Transduction/immunology
MH  - Syk Kinase
MH  - Transplantation Chimera/genetics/immunology
EDAT- 2003/01/09 04:00
MHDA- 2003/03/05 04:00
CRDT- 2003/01/09 04:00
PHST- 2003/01/09 04:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2003/01/09 04:00 [entrez]
AID - 10.4049/jimmunol.170.2.846 [doi]
PST - ppublish
SO  - J Immunol. 2003 Jan 15;170(2):846-52. doi: 10.4049/jimmunol.170.2.846.