PMID- 12519626
OWN - NLM
STAT- MEDLINE
DCOM- 20030611
LR  - 20191106
IS  - 0898-2104 (Print)
IS  - 0898-2104 (Linking)
VI  - 12
IP  - 4
DP  - 2002 Nov
TI  - The application of confocal microscopy to the study of liposome adsorption onto 
      bacterial biofilms.
PG  - 285-300
AB  - Confocal laser scanning microscopy has been used to visualise the adsorption of 
      fluorescently labelled liposomes on immobilised biofilms of the bacterium 
      Staphylococcus aureus. The liposomes were prepared with a wide range of 
      compositions with phosphatidylcholines as the predominant lipids using the 
      extrusion technique. They had weight average diameters of 125 +/- 5 nm and were 
      prepared with encapsulated carboxyfluorescein. Cationic liposomes were prepared 
      by incorporating dimethyldioctadecylammonium bromide (DDAB) or 3, beta [N-(N1,N1 
      dimethylammonium ethane)-carbamoyl] cholesterol (DC-chol) and anionic liposomes 
      were prepared by incorporation of phosphatidylinositol (PI). Pegylated cationic 
      liposomes were prepared by incorporation of DDAB and 
      1,2-dipalmitoylphosphatidylethanolamine-N-[polyethylene glycol)-2000]. Confocal 
      laser scanned images showed the preferential adsorption of the fluorescent 
      cationic liposomes at the biofilm-bulk phase interface which on quantitation gave 
      fluorescent peaks at the interface when scanned perpendicular (z-direction) to 
      the biofilm surface (x-y plane). The biofilm fluorescence enhancement (BFE) at 
      the interface was examined as a function of liposomal lipid concentration and 
      liposome composition. Studies of the extent of pegylation of the cationic 
      liposomes incorporating DDAB, on adsorption at the biofilm-bulk phase interface 
      were made. The results demonstrated that pegylation inhibited adsorption to the 
      bacterial biofilms as seen by the decline in the peak of fluorescence as the 
      mole% DPPE-PEG-2000 was increased in a range from 0 to 9 mole%. The results 
      indicate that confocal laser scanning microscopy is a useful technique for the 
      study of liposome adsorption to bacterial biofilms and complements the method 
      based on the use of radiolabelled liposomes.
FAU - Ahmed, Khalid
AU  - Ahmed K
AD  - School of Biological Sciences, University of Manchester, Manchester, M13 9PL, UK.
FAU - Gribbon, Phillip N
AU  - Gribbon PN
FAU - Jones, Malcolm N
AU  - Jones MN
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Liposome Res
JT  - Journal of liposome research
JID - 9001952
RN  - 0 (Fluoresceins)
RN  - 0 (Liposomes)
RN  - 0 (Phosphatidylcholines)
RN  - 3301-79-9 (6-carboxyfluorescein)
SB  - IM
MH  - Adsorption
MH  - *Biofilms
MH  - Fluoresceins
MH  - Fluorescence
MH  - Liposomes/chemistry/*metabolism
MH  - Microscopy, Confocal/*methods
MH  - Phosphatidylcholines
MH  - Staphylococcus aureus/*growth & development/*metabolism
EDAT- 2003/01/10 04:00
MHDA- 2003/06/12 05:00
CRDT- 2003/01/10 04:00
PHST- 2003/01/10 04:00 [pubmed]
PHST- 2003/06/12 05:00 [medline]
PHST- 2003/01/10 04:00 [entrez]
AID - 120016195 [pii]
AID - 10.1081/lpr-120016195 [doi]
PST - ppublish
SO  - J Liposome Res. 2002 Nov;12(4):285-300. doi: 10.1081/lpr-120016195.