PMID- 12520365
OWN - NLM
STAT- MEDLINE
DCOM- 20030506
LR  - 20061115
IS  - 0343-8651 (Print)
IS  - 0343-8651 (Linking)
VI  - 46
IP  - 2
DP  - 2003 Feb
TI  - Porphyromonas gingivalis modulates the production of interleukin 8 and monocyte 
      chemotactic protein 1 in human vascular endothelial cells.
PG  - 109-14
AB  - Porphyromonas gingivalis seems to perturb the cytokine network to maintain 
      periodontal disease. Although endothelial cells are a leading source of 
      interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1), the effect of P. 
      gingivalis on the cells remains unclear. We used reverse transcription-PCR 
      (RT-PCR) to assess levels of IL-8 and MCP-1 mRNA and enzyme-linked immunosorbent 
      assays (ELISA) to evaluate their protein production by human umbilical vein 
      endothelial cells (HUVEC) challenged with P. gingivalis. IL-8 and MCP-1 protein 
      levels decreased in response to challenge with P. gingivalis, and N-a- p-tosyl- 
      L-lysine chloromethylketone (TLCK) prevented the degradation of these chemokines. 
      Furthermore, the bacteria upregulated expression of the mRNAs of these 
      chemokines. Our results indicate that P. gingivalis proteases degraded IL-8 and 
      MCP-1. Degradation of chemokines secreted from endothelial cells may decrease the 
      recruitment of leukocytes and their migration through the endothelium, thus 
      contributing to a delay in the host immune defense mechanism.
FAU - Kobayashi-Sakamoto, Michiyo
AU  - Kobayashi-Sakamoto M
AD  - Department of Preventive Dentistry, Health Sciences University of Hokkaido, 
      Ishikari-Tobetsu, Hokkaido 061-0293, Japan. mkoba@hokuiryo-u.ac.jp
FAU - Isogai, Emiko
AU  - Isogai E
FAU - Hirose, Kimiharu
AU  - Hirose K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Endothelium, Vascular/*cytology/*microbiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-8/*biosynthesis
MH  - Porphyromonas gingivalis/*pathogenicity
MH  - RNA, Messenger/metabolism
MH  - Umbilical Veins
EDAT- 2003/01/10 04:00
MHDA- 2003/05/07 05:00
CRDT- 2003/01/10 04:00
PHST- 2003/01/10 04:00 [pubmed]
PHST- 2003/05/07 05:00 [medline]
PHST- 2003/01/10 04:00 [entrez]
AID - 10.1007/s00284-002-3782-x [doi]
PST - ppublish
SO  - Curr Microbiol. 2003 Feb;46(2):109-14. doi: 10.1007/s00284-002-3782-x.