PMID- 12522068
OWN - NLM
STAT- MEDLINE
DCOM- 20030715
LR  - 20181113
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 138
IP  - 1
DP  - 2003 Jan
TI  - Up-regulation of kinin B1 receptor in the lung of streptozotocin-diabetic rat: 
      autoradiographic and functional evidence.
PG  - 13-22
AB  - 1 The function and autoradiographic binding expression of kinin B(1) receptors 
      were evaluated in the lungs of Streptozotocin (STZ)-diabetic rats. 2 The 
      intrapleural injection (i.pl.) of des-Arg(9)-bradykinin (des-Arg(9)-BK) (50 and 
      100 nmol per site), a selective B(1) receptor agonist, increased time-dependently 
      the mononuclear and neutrophil cells influx in the pleural cavity of rats treated 
      with STZ (65 mg kg(-1), i.p., 4 days earlier). This effect was significantly less 
      in control rats. 3 The influx of mononuclear and polymorphonuclear neutrophil 
      cells induced by des-Arg(9)-BK was significantly inhibited by two B(1) receptor 
      antagonists (des-Arg(10)-Hoe140 or R-715, 100 nmol per site, 5 min earlier), but 
      not by two B(2) receptor antagonists (Hoe140, 10 nmol or NPC 18884, 100 nmol per 
      site, 5 min earlier). However, Hoe140 prevented the higher basal leukocyte influx 
      seen in STZ-diabetic rats. 4 Leukocyte infiltration induced by des-Arg(9)-BK in 
      STZ-diabetic rats was significantly reduced after treatment with insulin (2 U per 
      day, s.c. over 4 days) or with an anti-PMN antibody (0.1 ml of a 1 : 20 dilution, 
      i.pl. 5 min earlier). 5 Specific B(1) receptor binding sites were seen in lung 
      sections from both control and STZ-diabetic rats, yet the density of labelling 
      was much greater in diabetic rats and particularly after intrapleural injection 
      of des-Arg(9)-BK. Treatment with insulin or with the anti-PMN antibody markedly 
      reduced B(1) receptor binding sites occurring after the injection of 
      des-Arg(9)-BK in STZ-diabetic rats. 6 Data suggest that the B(1) receptor is 
      up-regulated in the lungs of STZ-diabetic rats, and its activation increases 
      leukocyte infiltration into the pleural cavity. The overexpression of B(1) 
      receptors seems to depend on neutrophils influx and appears to be associated with 
      hyperglycaemia.
FAU - Vianna, Rose Mari J
AU  - Vianna RM
AD  - Department of Physiology, Faculty of Medicine, Universite de Montreal, Montreal, 
      C.P. 6128, Succursale centre-ville, Montreal, Quebec, Canada H3C 3J7.
FAU - Ongali, Brice
AU  - Ongali B
FAU - Regoli, Domenico
AU  - Regoli D
FAU - Calixto, Joao Batista
AU  - Calixto JB
FAU - Couture, Rejean
AU  - Couture R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Receptor, Bradykinin B1)
RN  - 0 (Receptors, Bradykinin)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Cell Movement/drug effects/physiology
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Leukocytes/cytology/drug effects/metabolism
MH  - Lung/cytology/drug effects/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Bradykinin B1
MH  - Receptors, Bradykinin/agonists/*biosynthesis
MH  - Up-Regulation/drug effects/*physiology
PMC - PMC1573626
EDAT- 2003/01/11 04:00
MHDA- 2003/07/16 05:00
PMCR- 2004/01/01
CRDT- 2003/01/11 04:00
PHST- 2003/01/11 04:00 [pubmed]
PHST- 2003/07/16 05:00 [medline]
PHST- 2003/01/11 04:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 0704999 [pii]
AID - 10.1038/sj.bjp.0704999 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2003 Jan;138(1):13-22. doi: 10.1038/sj.bjp.0704999.