PMID- 12524060
OWN - NLM
STAT- MEDLINE
DCOM- 20030130
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 79
IP  - 1
DP  - 2003 Jan
TI  - Chromosomal abnormalities in embryos derived from testicular sperm extraction.
PG  - 30-8
AB  - OBJECTIVE: To compare the rate of chromosome abnormalities in embryos obtained 
      from karyotypically normal patients with nonobstructive azoospermia undergoing 
      testicular sperm extraction (TESE) to those from patients undergoing 
      intracytoplasmic sperm injection (ICSI) with ejaculated sperm. DESIGN: 
      Retrospective analysis. SETTING: IVF centers. PATIENT(S): Male partners had 
      either nonobstructive zoospermia or oligospermia. INTERVENTION(S): 
      Preimplantation genetic diagnosis. Chromosome enumeration was performed by 
      fluorescence in situ hybridization (FISH). Embryos classified as abnormal were 
      reanalyzed to study mosaicism. MAIN OUTCOME MEASURE(S): Chromosome abnormalities 
      in embryos. RESULT(S): Embryos from ICSI cycles with ejaculated sperm (group 1) 
      were 41.8% normal, 26.2% aneuploid, and 26.5% mosaic. In contrast, the embryos 
      from ICSI cycles with TESE for nonobstructive azoospermia (group 2) were 22% 
      normal, 17% aneuploid, and 53% mosaic. The difference in mosaicism rate between 
      the two groups of embryos was highly significant. CONCLUSION(S): The present 
      study results indicate a high incidence of mosaicism in embryos derived from TESE 
      in men with a severe deficit in spermatogenesis. Sperm derived from TESE for 
      nonobstructive azoospermia may have a higher rate of compromised or immature 
      centrosome structures leading to mosaicism in the embryo.
FAU - Silber, Sherman
AU  - Silber S
AD  - Infertility Center of St. Louis at St. Luke's Hospital, St. Louis, Missouri, USA.
FAU - Escudero, Tomas
AU  - Escudero T
FAU - Lenahan, Kathleen
AU  - Lenahan K
FAU - Abdelhadi, Iman
AU  - Abdelhadi I
FAU - Kilani, Zaid
AU  - Kilani Z
FAU - Munne, Santiago
AU  - Munne S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
CIN - J Urol. 2003 Aug;170(2 Pt 1):676. PMID: 14601567
MH  - Aneuploidy
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mosaicism
MH  - Oligospermia/therapy
MH  - *Preimplantation Diagnosis
MH  - Retrospective Studies
MH  - *Sperm Injections, Intracytoplasmic
MH  - *Spermatozoa
MH  - Testis/*cytology
MH  - Tissue and Organ Harvesting/*methods
EDAT- 2003/01/14 04:00
MHDA- 2003/01/31 04:00
CRDT- 2003/01/14 04:00
PHST- 2003/01/14 04:00 [pubmed]
PHST- 2003/01/31 04:00 [medline]
PHST- 2003/01/14 04:00 [entrez]
AID - S0015028202044072 [pii]
AID - 10.1016/s0015-0282(02)04407-2 [doi]
PST - ppublish
SO  - Fertil Steril. 2003 Jan;79(1):30-8. doi: 10.1016/s0015-0282(02)04407-2.