PMID- 12525872
OWN - NLM
STAT- MEDLINE
DCOM- 20030812
LR  - 20041117
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 11
IP  - 2
DP  - 2003 Feb
TI  - Impaired function of antigen-presenting dendritic cells in patients with chronic 
      hepatitis B: localization of HBV DNA and HBV RNA in blood DC by in situ 
      hybridization.
PG  - 169-74
AB  - Antigen-presenting dendritic cells (DCs), which play a major role in the 
      triggering of primary anti viral immune reactions, may also contribute, in some 
      viral models, to the pathogenesis of persistent viral infection. In fact, 
      impaired immune response to hepatitis B virus (HBV)-encoded antigens is seen in 
      patients with chronic hepatitis B (CH-B). The aim of this study was to check the 
      function of DCs in these patients and to investigate the underlying mechanism. 
      DCs were enriched from peripheral blood mononuclear cells by culturing with 
      interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor for 7 
      days. The stimulatory capacity of DCs were checked in allogenic mixed leukocyte 
      (MLR) reaction. The levels of IL-12 in the culture supernatants were measured by 
      an enzyme-linked immunosorbent assay. HBV DNA and HBV RNA were localized in DCs 
      by polymerase chain reaction (PCR) in situ hybridization and 
      reverse-transcriptase (RT)-PCR in situ hybridization. The stimulatory capacity of 
      DCs in allogenic MLR was significantly lower in patients with CH-B (36321+/-12523 
      cpm, n=18) compared to that of normal controls (65678+/-11174 cpm, n=18) 
      (p<0.0001). Significantly lower levels of IL-12 were detected in cultures 
      containing DCs from patients with CH-B than normal controls (46.7+/-25.6 versus 
      122.4+/- 37.1 pg/ml, p<0.0001). In situ hybridization revealed the localization 
      of HBV DNA and HBV RNA in DCs from patients with CH-B. These results indicate 
      that chronic infection by HBV is associated with functional defects of DCs. 
      Localization of HBV DNA and HBV RNA indicates that DCs may constitute an extra 
      hepatic reservoir and possibly of replication of HBV.
FAU - Arima, Shouko
AU  - Arima S
AD  - Third Department of Internal Medicine, Ehime University School of Medicine, 
      Shigenobu-Cho, Ehime 791-0295, Japan.
FAU - Akbar, S M Fazle
AU  - Akbar SM
FAU - Michitaka, Kojiro
AU  - Michitaka K
FAU - Horiike, Norio
AU  - Horiike N
FAU - Nuriya, Hideko
AU  - Nuriya H
FAU - Kohara, Michinori
AU  - Kohara M
FAU - Onji, Morikazu
AU  - Onji M
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 187348-17-0 (Interleukin-12)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Antigen-Presenting Cells/*immunology
MH  - Chronic Disease
MH  - DNA/blood
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Hepatitis B/*immunology
MH  - Hepatitis B virus/*genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Interleukin-12/blood
MH  - Male
MH  - RNA/blood
EDAT- 2003/01/15 04:00
MHDA- 2003/08/13 05:00
CRDT- 2003/01/15 04:00
PHST- 2003/01/15 04:00 [pubmed]
PHST- 2003/08/13 05:00 [medline]
PHST- 2003/01/15 04:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2003 Feb;11(2):169-74.