PMID- 12526794
OWN - NLM
STAT- MEDLINE
DCOM- 20030214
LR  - 20220309
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 112
IP  - 1
DP  - 2003 Jan 10
TI  - Hippocampal neuronal polarity specified by spatially localized mPar3/mPar6 and PI 
      3-kinase activity.
PG  - 63-75
AB  - How a neuron becomes polarized remains an outstanding question. Here, we report 
      that selection of the future axon among neurites of a cultured hippocampal neuron 
      requires the activity of growth factor receptor tyrosine kinase, 
      phosphatidylinositol 3-kinase (PI 3-kinase), as well as atypical protein kinase C 
      (aPKC). The PI 3-kinase activity, highly localized to the tip of the newly 
      specified axon of stage 3 neurons, is essential for the proper subcellular 
      localization of mPar3, the mammalian homolog of C. elegans polarity protein Par3. 
      Polarized distribution of not only mPar3 but also mPar6 is important for axon 
      formation; ectopic expression of mPar6 or mPar3, or just the N terminus of mPar3, 
      leaves neurons with no axon specified. Thus, neuronal polarity is likely to be 
      controlled by the mPar3/mPar6/aPKC complex and the PI 3-kinase signaling pathway, 
      both serving evolutionarily conserved roles in specifying cell polarity.
FAU - Shi, Song-Hai
AU  - Shi SH
AD  - Howard Hughes Medical Institute, Department of Physiology, University of 
      California at San Francisco, 533 Parnassus Avenue, San Francisco, CA 94143, USA.
FAU - Jan, Lily Yeh
AU  - Jan LY
FAU - Jan, Yuh-Nung
AU  - Jan YN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Proteins)
RN  - 0 (Receptors, Thrombin)
RN  - 0 (protease-activated receptor 3)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
CIN - Cell. 2003 Jan 10;112(1):4-5. PMID: 12526787
MH  - Animals
MH  - *Cell Polarity
MH  - Cells, Cultured
MH  - Hippocampus/*cytology
MH  - Models, Biological
MH  - Mutation
MH  - Neurons/cytology/enzymology/*metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Protein Kinase C/metabolism
MH  - Proteins/chemistry/*metabolism
MH  - Rats
MH  - Receptors, Thrombin/chemistry/*metabolism
MH  - Signal Transduction
EDAT- 2003/01/16 04:00
MHDA- 2003/02/15 04:00
CRDT- 2003/01/16 04:00
PHST- 2003/01/16 04:00 [pubmed]
PHST- 2003/02/15 04:00 [medline]
PHST- 2003/01/16 04:00 [entrez]
AID - S0092867402012497 [pii]
AID - 10.1016/s0092-8674(02)01249-7 [doi]
PST - ppublish
SO  - Cell. 2003 Jan 10;112(1):63-75. doi: 10.1016/s0092-8674(02)01249-7.