PMID- 12526888
OWN - NLM
STAT- MEDLINE
DCOM- 20030822
LR  - 20211102
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 33
IP  - 1
DP  - 2003 Jan
TI  - Disturbances in purinergic [Ca2+]i signaling pathways in a transformed rat 
      thyroid cell line.
PG  - 59-68
AB  - It was previously shown that in rat thyroid PC-Cl3 cell line, a purinergic P2Y 
      receptor increases the concentration of free cytosolic Ca(2+) ([Ca(2+)](i)) via 
      phospholipase C activation. We here studied whether in a transformed cell line 
      (PC-E1Araf) derived from parental PC-Cl3 cells, ATP is still able to transduce 
      the [Ca(2+)](i)-based intracellular signal.We demonstrate the expression of mRNA 
      for P2Y2 in both PC-Cl3 and PC-E1Araf cells; mRNAs for P2Y1, P2Y4, P2Y6 and P2Y11 
      were absent. In both cell lines activation of P2Y2 receptor provokes a transient 
      increase in [Ca(2+)](i) followed by a lower sustained phase persisting for over 
      5min in PC-Cl3 and only 1.5 min in PC-E1Araf cells. In both cell lines the 
      [Ca(2+)](i) reached a plateau level significantly higher than the basal 
      [Ca(2+)](i) level persisting for over 10 min. Removal of extracellular Ca(2+) 
      reduced the initial transient response to ATP in PC-Cl3, but not in PC-E1Araf 
      cells, and completely abolished the plateau phase in both cell lines. In the 
      presence of extracellular Ca(2+) thapsigargin (TG) caused a rise in [Ca(2+)](i) 
      significantly higher in PC-Cl3 than transformed PC-E1Araf cells, while in 
      Ca(2+)-free medium the effect of TG was similar in both cell lines. The 
      capacitative Ca(2+)-entry in PC-Cl3 resulted significantly higher than in 
      PC-E1Araf cells. Further studies were performed in order to investigate whether 
      the different effects of ATP on [Ca(2+)](i) was due to variation in divalent 
      cation plasma membrane permeability. PC-E1Araf cells showed a much lower 
      permeability to Ca(2+), Ba(2+), Sr(2+), Mn(2+), and Co(2+) that may be 
      responsible for the differences in purinergic Ca(2+) signaling pathway with 
      respect to parental PC-Cl3 cells.
FAU - Elia, Maria Giovanna
AU  - Elia MG
AD  - Laboratorio di Fisiologia, Dipartimento di Scienze e Tecnologie Biologiche e 
      Ambientali, Universita di Lecce, 73100 Lecce, Italy.
FAU - Muscella, Antonella
AU  - Muscella A
FAU - Greco, Simona
AU  - Greco S
FAU - Vilella, Sebastiano
AU  - Vilella S
FAU - Storelli, Carlo
AU  - Storelli C
FAU - Marsigliante, Santo
AU  - Marsigliante S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Cations)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (P2ry2 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2Y2)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism/pharmacology
MH  - Animals
MH  - Calcium/deficiency
MH  - Calcium Signaling/drug effects/*physiology
MH  - Cations/metabolism/pharmacology
MH  - Cell Line, Transformed
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Membrane Permeability/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Epithelial Cells/drug effects/*metabolism
MH  - Extracellular Space/drug effects/metabolism
MH  - Gene Expression/drug effects/physiology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Receptors, Purinergic P2/drug effects/genetics/*metabolism
MH  - Receptors, Purinergic P2Y2
MH  - Thyroid Gland/drug effects/*metabolism
MH  - Up-Regulation/drug effects/physiology
EDAT- 2003/01/16 04:00
MHDA- 2003/08/23 05:00
CRDT- 2003/01/16 04:00
PHST- 2003/01/16 04:00 [pubmed]
PHST- 2003/08/23 05:00 [medline]
PHST- 2003/01/16 04:00 [entrez]
AID - S0143416002001963 [pii]
AID - 10.1016/s0143-4160(02)00196-3 [doi]
PST - ppublish
SO  - Cell Calcium. 2003 Jan;33(1):59-68. doi: 10.1016/s0143-4160(02)00196-3.