PMID- 12527064
OWN - NLM
STAT- MEDLINE
DCOM- 20030409
LR  - 20190822
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 59
IP  - 5-6
DP  - 2003 Mar
TI  - Fertility of ram semen frozen in Bioexcell and used for cervical artificial 
      insemination.
PG  - 1157-70
AB  - The current use of ingredients of animal origin, such as egg yolk, in semen 
      extenders presents a risk of microbial contamination, and has led to the search 
      for alternatives. Such an extender is commercially available for bull semen 
      (Bioexcell), IMV, L'Aigle, France), and it has previously been tested in vitro 
      for freezing ram semen, with satisfactory results. The aim of the present study 
      was to compare the fertility results of ewes in Uruguay, after cervical 
      insemination with ram semen that was frozen in Bioexcell versus semen frozen in a 
      conventional milk-egg yolk extender (control). Semen from five Corriedale rams 
      was frozen, using a split sample design, in either milk-egg yolk or Bioexcell 
      extender, using a two-step extension method. The sperm parameters assessed after 
      thawing were subjective motility, membrane integrity (SYBR-14/PI), and 
      capacitation status (CTC). Thawed semen was inseminated intracervically once 
      during spontaneous estrus in 970 Corriedale ewes that grazed in natural pastures, 
      under extensive management conditions. Fertility was recorded as nonreturn rates 
      at 21 days (NRR-21) and 36 days (NRR-36) after artificial insemination (AI), as 
      well as pregnancy rate (PR-US, diagnosed ultrasonographically 50 days after AI of 
      the last ewe). Subjective motility was slightly higher in Bioexcell than in the 
      milk extender (47 vs. 46.5%; NS), as was membrane integrity (38 vs. 37.7%; NS) 
      and the percentage of uncapacitated spermatozoa (28.5 vs. 26.3%; NS). There were 
      no statistically significant differences in fertility rates found between 
      Bioexcell and the control extender: NRR-21 (35.9 vs. 33.2%), NRR-36 (34.8 vs. 
      32.6%), and PR-US (28.4 vs. 27.2%). In conclusion, Bioexcell appears to be an 
      alternative to the conventional milk-egg yolk extender for freezing ram semen, 
      and provides similar fertility results after cervical AI under extensive 
      management conditions. Thus, Bioexcell, containing no additives of animal origin, 
      can offer a safer alternative when frozen semen is used for introducing new 
      genetic material into a flock or a country.
FAU - Gil, J
AU  - Gil J
AD  - Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Swedish 
      University of Agricultural Sciences (SLU), Ullsvagen 14C, Clinical Center, PO Box 
      7039, Ultuna, SE-750 07, Uppsala, Sweden.
FAU - Rodriguez-Irazoqui, M
AU  - Rodriguez-Irazoqui M
FAU - Lundeheim, N
AU  - Lundeheim N
FAU - Soderquist, L
AU  - Soderquist L
FAU - Rodriguez-Martinez, H
AU  - Rodriguez-Martinez H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Cryoprotective Agents)
RN  - WCK1KIQ23Q (Chlortetracycline)
SB  - IM
MH  - Animals
MH  - Cell Membrane/physiology
MH  - Chlortetracycline/metabolism
MH  - Cryopreservation/methods/*veterinary
MH  - Cryoprotective Agents/metabolism
MH  - Female
MH  - Fertility/*physiology
MH  - Insemination, Artificial/methods/veterinary
MH  - Male
MH  - Microscopy, Fluorescence/veterinary
MH  - Pregnancy
MH  - Random Allocation
MH  - Semen Preservation/methods/*veterinary
MH  - Sheep/*physiology
MH  - Sperm Capacitation/physiology
MH  - Sperm Motility/physiology
MH  - Spermatozoa/*physiology
MH  - Uruguay
EDAT- 2003/01/16 04:00
MHDA- 2003/04/10 05:00
CRDT- 2003/01/16 04:00
PHST- 2003/01/16 04:00 [pubmed]
PHST- 2003/04/10 05:00 [medline]
PHST- 2003/01/16 04:00 [entrez]
AID - S0093691X02011780 [pii]
AID - 10.1016/s0093-691x(02)01178-0 [doi]
PST - ppublish
SO  - Theriogenology. 2003 Mar;59(5-6):1157-70. doi: 10.1016/s0093-691x(02)01178-0.