PMID- 12527074 OWN - NLM STAT- MEDLINE DCOM- 20030409 LR - 20190822 IS - 0093-691X (Print) IS - 0093-691X (Linking) VI - 59 IP - 5-6 DP - 2003 Mar TI - Factors affecting the survival of sheep embryos after transfer within a MOET program. PG - 1265-75 AB - Multiple ovulation and embryo transfer (MOET) has the potential to increase the rate of genetic improvement in sheep. However, better realization of this potential requires maximum survival rates of transferred embryos of high genetic merit after transfer into recipient ewes. These studies were therefore conducted to investigate the effect of both embryonic and recipient ewe factors on the survival rate of transferred embryos. Survival rate was similar after transfer of morula or blastocyst stage embryos, and these were higher (P<0.05) than for very early morulae and early morulae. Advanced embryos (Day 5 blastocyst) had an advantage (P<0.05) in survival rate over retarded embryos (Day 6 morula). Grades 1 and 2 embryos survived significantly (P<0.05) better than Grades 3 or 4 embryos. There was no difference in embryo survival rate following transfer to recipients with different numbers of corpora lutea. In general, age or parity of recipient ewes did not affect embryo survival rate, although a higher (P<0.05) embryo survival rate was observed for yearling recipients. Buserelin (GnRH agonist) treatment of recipient ewes 5 or 6 days after transfer of embryos (Day 12 of the cycle) did not improve embryo survival rate. These results confirm that both embryonic and recipient factors can play an important role in the success of a MOET program in sheep. FAU - Bari, F AU - Bari F AD - Institute of Rural Studies, University of Wales Aberystwyth, Llanbadarn Campus, Aberystwyth, Ceredigion SY23 3AL, UK. FAU - Khalid, M AU - Khalid M FAU - Haresign, W AU - Haresign W FAU - Murray, A AU - Murray A FAU - Merrell, B AU - Merrell B LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Theriogenology JT - Theriogenology JID - 0421510 RN - 0 (Fertility Agents, Female) RN - PXW8U3YXDV (Buserelin) SB - IM MH - Age Factors MH - Animals MH - Buserelin/pharmacology MH - Corpus Luteum/physiology MH - Embryo Transfer/*veterinary MH - Embryonic and Fetal Development/physiology MH - Female MH - Fertility Agents, Female/pharmacology MH - Fetal Death/*veterinary MH - Male MH - Pregnancy MH - Retrospective Studies MH - Sheep/*embryology/physiology EDAT- 2003/01/16 04:00 MHDA- 2003/04/10 05:00 CRDT- 2003/01/16 04:00 PHST- 2003/01/16 04:00 [pubmed] PHST- 2003/04/10 05:00 [medline] PHST- 2003/01/16 04:00 [entrez] AID - S0093691X02011627 [pii] AID - 10.1016/s0093-691x(02)01162-7 [doi] PST - ppublish SO - Theriogenology. 2003 Mar;59(5-6):1265-75. doi: 10.1016/s0093-691x(02)01162-7.