PMID- 12528824
OWN - NLM
STAT- MEDLINE
DCOM- 20030424
LR  - 20220408
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 67
IP  - 1
DP  - 2003 Jan
TI  - Mechanical transmission of porcine reproductive and respiratory syndrome virus 
      throughout a coordinated sequence of events during warm weather.
PG  - 12-9
AB  - Mechanical transmission of porcine reproductive and respiratory syndrome virus 
      (PRRSV) throughout a coordinated sequence of events that replicated common farm 
      worker behavior during warm weather (10 degrees C to 16 degrees C) was assessed 
      using a field-based model. The model involved fomites (boots and containers), 
      vehicle sanitation, transport, and personnel movement. In a previous study, the 
      model successfully demonstrated mechanical transmission of PRRSV in 8 out of 10 
      replicates during cold weather. A field strain of PRRSV was inoculated into 
      carriers consisting of soil samples, which were adhered to the undercarriage of a 
      vehicle. The vehicle was driven approximately 50 km to a commercial truck washing 
      facility where the driver's boots contacted the carriers during washing, 
      introducing the virus to the vehicle interior. The vehicle was then driven 50 km 
      to a simulated farm site, and the driver's boots mechanically spread virus into 
      the farm anteroom. Types of containers frequently employed in swine farms 
      contacted drippings from the footwear on the anteroom floor. The truck wash 
      floor, vehicle cab floor mats, boot soles, anteroom floor, and the ventral 
      surface of containers were sampled to track the virus throughout the model. Ten 
      replicates were conducted, along with sham-inoculated controls, and control 
      replicates. In 2 replicates, infectious PRRSV was detected on the anteroom floor 
      and in 1 replicate, infectious PRRSV was detected on the surface of the container 
      by swine bioassay. All sham-inoculated controls and protocol controls were 
      negative. These results indicate that mechanical transmission of PRRSV throughout 
      a coordinated sequence of events in warm weather can occur, but in contrast to 
      data from studies conducted during cold weather, it appears to be a relatively 
      infrequent event.
FAU - Dee, Scott
AU  - Dee S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, St. Paul, Minnesota 55108, USA. deexx004@umn.edu
FAU - Deen, John
AU  - Deen J
FAU - Rossow, Kurt
AU  - Rossow K
FAU - Weise, Carrie
AU  - Weise C
FAU - Eliason, Roger
AU  - Eliason R
FAU - Otake, Satoshi
AU  - Otake S
FAU - Joo, Han Soo
AU  - Joo HS
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (DNA, Viral)
RN  - 0 (Pharmaceutical Vehicles)
SB  - IM
MH  - Animal Husbandry
MH  - Animals
MH  - Biological Assay/veterinary
MH  - DNA, Viral/isolation & purification
MH  - *Disease Reservoirs
MH  - Minnesota
MH  - Models, Biological
MH  - Pharmaceutical Vehicles
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification/*pathogenicity
MH  - Seasons
MH  - Shoes
MH  - Swine
MH  - Temperature
PMC - PMC227022
EDAT- 2003/01/17 04:00
MHDA- 2003/04/25 05:00
PMCR- 2003/04/01
CRDT- 2003/01/17 04:00
PHST- 2003/01/17 04:00 [pubmed]
PHST- 2003/04/25 05:00 [medline]
PHST- 2003/01/17 04:00 [entrez]
PHST- 2003/04/01 00:00 [pmc-release]
AID - 0000000-200301000-00002 [pii]
PST - ppublish
SO  - Can J Vet Res. 2003 Jan;67(1):12-9.