PMID- 12529394
OWN - NLM
STAT- MEDLINE
DCOM- 20030225
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 23
IP  - 3
DP  - 2003 Feb
TI  - Nuclear factor 1 is required for both hormone-dependent chromatin remodeling and 
      transcriptional activation of the mouse mammary tumor virus promoter.
PG  - 887-98
AB  - The mouse mammary tumor virus (MMTV) promoter has been used as a model to study 
      how the glucocorticoid receptor (GR) remodels chromatin to allow other 
      transcription factors to bind and activate transcription. To dissect the precise 
      role of nuclear factor 1 (NF1) in chromatin remodeling and transcriptional 
      activation, we used linker-scanning mutants of transcription factor binding sites 
      on the MMTV promoter. We compared the NF1 mutant MMTV promoter in the context of 
      transiently transfected templates (transient transfection) and templates 
      organized as chromatin (stable transfection) to understand the effect of 
      chromatin on factor binding and transcription. We show that on a transiently 
      transfected template, mutation in the NF1 binding site reduces both basal and 
      hormone-dependent transcription. This suggests that NF1 is required for 
      transcription in the absence of organized chromatin. We also found that binding 
      of NF1 on a transiently transfected template is independent of mutation in 
      hormone response elements or the octamer transcription factor (OTF) binding site. 
      In contrast, the binding of OTF proteins to a transiently transfected template 
      was found to be dependent on the binding of NF1, which may imply that NF1 has a 
      stabilizing effect on OTF binding. On a chromatin template, mutation in the NF1 
      binding site does not affect the positioning of nucleosomes on the promoter. We 
      also show that in the absence of NF1 binding, GR-mediated chromatin remodeling of 
      nucleosome B is reduced and hormone-dependent activation of transcription is 
      abolished. Further, we demonstrate that NF1 is required for both the association 
      of BRG1 chromatin remodeling complex and the GR on the promoter in vivo. These 
      results suggest the novel possibility that NF1 may participate in chromatin 
      remodeling activities in addition to directly enhancing transcription and that in 
      the absence of its binding site the GR is unable to effectively bind the promoter 
      and recruit the remodeling complex.
FAU - Hebbar, Pratibha B
AU  - Hebbar PB
AD  - Chromatin and Gene Expression Section, Laboratory of Reproductive and 
      Developmental Toxicology, National Institute of Environmental Health Science, 
      Research Triangle Park, North Carolina 27709, USA.
FAU - Archer, Trevor K
AU  - Archer TK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - CCAAT-Enhancer-Binding Proteins/genetics/*metabolism
MH  - Cell Line
MH  - Chromatin/*genetics/*metabolism
MH  - DNA, Viral/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Genes, Viral
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Plasmids/genetics
MH  - Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Transcriptional Activation
MH  - Transfection
MH  - Y-Box-Binding Protein 1
PMC - PMC140717
EDAT- 2003/01/17 04:00
MHDA- 2003/02/26 04:00
PMCR- 2003/02/01
CRDT- 2003/01/17 04:00
PHST- 2003/01/17 04:00 [pubmed]
PHST- 2003/02/26 04:00 [medline]
PHST- 2003/01/17 04:00 [entrez]
PHST- 2003/02/01 00:00 [pmc-release]
AID - 1383 [pii]
AID - 10.1128/MCB.23.3.887-898.2003 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2003 Feb;23(3):887-98. doi: 10.1128/MCB.23.3.887-898.2003.