PMID- 12529690
OWN - NLM
STAT- MEDLINE
DCOM- 20030220
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 17
IP  - 1
DP  - 2003 Jan
TI  - Is t(14;18)(q32;q21) a constant finding in follicular lymphoma? An interphase 
      FISH study on 63 patients.
PG  - 255-9
AB  - The translocation (14;18)(q32;q21) is the hallmark of follicular lymphoma (FL). 
      However, conventional cytogenetics and PCR techniques fail to detect it in at 
      least 10% of cases. In order to evaluate the true incidence of this translocation 
      in FL, we analyzed 63 patients with FL, and 17 patients with diffuse large cell 
      lymphoma (DLCL) corresponding to suspected FL transformations using interphase 
      fluorescence in situ hybridization (FISH). Colocalized signals related to the 
      translocation were observed in 19-92% of cells (median = 51%), corresponding to 
      positivity over the threshold in all (63/63) cases. Similarly, 16/17 possibly 
      secondary DLCL displayed the translocation. Although some cytogenetic changes 
      might be missed by this FISH assay (such as rare insertion, or translocations 
      with other chromosomal partners), our results stress t(14;18)(q32;q21) as an 
      almost constant finding in FL. Our sensitive interphase FISH assay should be of 
      great value to define FL more accurately, namely in patients included into 
      therapeutic trials. Furthermore, this approach could be of interest in 
      (re)defining some types of FL, especially the grade 3 FL which frequently lack 
      t(14;18).
FAU - Godon, A
AU  - Godon A
AD  - Hematology Laboratory, University Hospital, Nantes, France.
FAU - Moreau, A
AU  - Moreau A
FAU - Talmant, P
AU  - Talmant P
FAU - Baranger-Papot, L
AU  - Baranger-Papot L
FAU - Genevieve, F
AU  - Genevieve F
FAU - Milpied, N
AU  - Milpied N
FAU - Zandecki, M
AU  - Zandecki M
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Chromosome Banding
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - France
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Lymphoma, Follicular/diagnosis/*genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/diagnosis/*genetics/pathology
MH  - Ploidies
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Retrospective Studies
MH  - Translocation, Genetic
EDAT- 2003/01/17 04:00
MHDA- 2003/02/21 04:00
CRDT- 2003/01/17 04:00
PHST- 2002/01/16 00:00 [received]
PHST- 2002/07/02 00:00 [accepted]
PHST- 2003/01/17 04:00 [pubmed]
PHST- 2003/02/21 04:00 [medline]
PHST- 2003/01/17 04:00 [entrez]
AID - 2402739 [pii]
AID - 10.1038/sj.leu.2402739 [doi]
PST - ppublish
SO  - Leukemia. 2003 Jan;17(1):255-9. doi: 10.1038/sj.leu.2402739.