PMID- 125303
OWN - NLM
STAT- MEDLINE
DCOM- 19751107
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 115
IP  - 3
DP  - 1975 Sep
TI  - Modulation of HL-A antigens by anti-HLA antiserum: effects on the cytotoxicity 
      assay and mixed leukocyte reaction.
PG  - 811-6
AB  - Modulation of human leukocyte antigen (HLA) was attempted by treating leukocytes 
      with specific anti-HLA antiserum or by their passage through columns coated with 
      anti-HLA or a double layer of HLA-anti-HLA. The modulated cells were resistant to 
      the cytotoxic effects of the anti-HLA, and they were poor stimulators and good 
      responders to allogeneic cells in the unidirectional mixed leukocyte reaction. 
      Modulated cells regained their HLA 16 hr after modulation if kept in cell 
      suspension alone. The proliferative responses of modulated cells to mitogens were 
      as good as non-modulated cells, indicating that modulation was probably not 
      caused by depletion of lymphoid cells. Supernatants of modulated cells that were 
      incubated overnight or preformed HLA-anti-HLA complexes were capable of 
      suppressing and enhancing the MLR of specific cells depending on the dose used. 
      The similarities of modulation of HLA to other lymphocyte receptors and the 
      limitation of application of the modulation phenomenon to transplantation of 
      allogeneic cells are discussed.
FAU - Sadeghee, S
AU  - Sadeghee S
FAU - Hebert, J
AU  - Hebert J
FAU - Kelley, J
AU  - Kelley J
FAU - Abdou, N I
AU  - Abdou NI
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens)
RN  - 0 (Isoantibodies)
RN  - 0 (Lectins)
RN  - 10028-17-8 (Tritium)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Antibody Specificity
MH  - Antigen-Antibody Complex
MH  - Cell Separation
MH  - Cell-Free System
MH  - Cells, Cultured
MH  - Cytotoxicity Tests, Immunologic
MH  - Glass
MH  - *HLA Antigens
MH  - Histocompatibility
MH  - *Histocompatibility Antigens
MH  - Humans
MH  - *Immunity, Cellular
MH  - *Isoantibodies
MH  - Lectins
MH  - Leukocytes/immunology
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Thymidine/metabolism
MH  - Tritium
EDAT- 1975/09/01 00:00
MHDA- 1975/09/01 00:01
CRDT- 1975/09/01 00:00
PHST- 1975/09/01 00:00 [pubmed]
PHST- 1975/09/01 00:01 [medline]
PHST- 1975/09/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1975 Sep;115(3):811-6.