PMID- 12530578
OWN - NLM
STAT- MEDLINE
DCOM- 20030620
LR  - 20041117
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 198
IP  - 11
DP  - 2002
TI  - Infection of murine precision cut lung slices (PCLS) with respiratory syncytial 
      virus (RSV) and chlamydophila pneumoniae using the Krumdieck technique.
PG  - 747-53
AB  - The Krumdieck technique allows the investigation of the so-called precision cut 
      lung slices (PCLS) with a special microtome. It is thus possible to evaluate 
      morphologic changes over a longer period of time using only a small group of 
      animals. Chlamydophila pneumoniae (Cp) and respiratory syncytial virus (RSV) 
      proved to be important causes of pneumonia, rhinitis and exacerbations of asthma 
      bronchiale, as well as of lower respiratory tract infections in young children. 
      PCLS should be tested for their suitability as an in vitro model for these 
      infections. The PCLS were infected with Cp and RSV over different periods of 
      time. Investigations were carried out by light and transmission electron 
      microscopy (TEM). Furthermore, immunofluorescence (IF) studies with antibodies 
      against bacterial or viral proteins and cell-specific markers were done using 
      confocal laser scanning microscopy (CLSM). Non-infected and infected PCLS showed 
      a well-preserved morphology up to 72 hours. After short infection intervals, 
      typical inclusions of Cp or RSV were detected in vacuoles of different cell 
      types. Infection and cell types could be verified using IF. Cytopathic effects 
      were not prominent. Ciliary beat was detectable up to 96 hours after infection. 
      This in vitro technique offers the possibility of studying mechanisms and effects 
      of bacterial and viral infections on viable tissue complexes.
FAU - Ebsen, M
AU  - Ebsen M
AD  - Department of Pathology, Ruhr-University Bochum, Universitatsstr. 150, 44801 
      Bochum, Germany. Michael.ebsen@ruhr-uni-bochum.de
FAU - Mogilevski, G
AU  - Mogilevski G
FAU - Anhenn, O
AU  - Anhenn O
FAU - Maiworm, V
AU  - Maiworm V
FAU - Theegarten, D
AU  - Theegarten D
FAU - Schwarze, J
AU  - Schwarze J
FAU - Morgenroth, K
AU  - Morgenroth K
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
SB  - IM
MH  - Animals
MH  - Chlamydophila Infections/*pathology
MH  - Chlamydophila pneumoniae
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Histological Techniques/methods
MH  - Lung/*microbiology/ultrastructure
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron
MH  - Microtomy/methods
MH  - Organ Culture Techniques
MH  - Respiratory Syncytial Virus Infections/*pathology
MH  - Respiratory Syncytial Viruses
EDAT- 2003/01/18 04:00
MHDA- 2003/06/21 05:00
CRDT- 2003/01/18 04:00
PHST- 2003/01/18 04:00 [pubmed]
PHST- 2003/06/21 05:00 [medline]
PHST- 2003/01/18 04:00 [entrez]
AID - S0344-0338(04)70329-5 [pii]
AID - 10.1078/0344-0338-00331 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2002;198(11):747-53. doi: 10.1078/0344-0338-00331.