PMID- 12532279
OWN - NLM
STAT- MEDLINE
DCOM- 20030930
LR  - 20161124
IS  - 0022-2631 (Print)
IS  - 0022-2631 (Linking)
VI  - 191
IP  - 1
DP  - 2003 Jan 1
TI  - Hepatocyte growth factor/scatter factor stimulates Ca2+-activated membrane K+ 
      current and migration of MDCK II cells.
PG  - 77-86
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) stimulates migration of various 
      cells and has been linked via Met tyrosine kinase-signaling to transformation and 
      the metastatic phenotype. Migration of transformed MDCK-F cells depends on 
      activation of a charybdotoxin-sensitive, volume-activated membrane K+ current. 
      Thus, we used patch-clamp electrophysiology and transwell migration assays to 
      determine whether HGF/SF stimulation of MDCK II cell migration depends on the 
      activation of membrane K+ currents. HGF/SF activated a membrane K+ current that 
      increased over 24 hr, and which could be modulated by increasing intracellular 
      calcium concentration, [Ca2+]i. Charybdotoxin (ChTX, 50 nM), iberiotoxin (IbTX, 
      100 nM), stichodactyla toxin (Stk, 100 nM) and clotrimazole (CLT, 1 mM) all 
      inhibited this current. HGF/SF (100 scatter units/ml) significantly increased 
      MDCK II cell migration over 8 hr compared to control cells. Addition of ChTX (50 
      nM), IbTX (100 nM), Stk (100 nM) or CLT (1 microM) inhibited the 
      HGF/SF-stimulated MDCK II cell migration. We conclude that the activation of 
      membrane Ca2+-activated K+current is necessary for HGF/SF stimulation of MDCK II 
      cell.
FAU - Jin, M
AU  - Jin M
AD  - Department of Physiology, James H. Quillen College of Medicine, East Tennessee 
      State University, PO Box 70,576, Johnson City, TN 37614, USA.
FAU - Defoe, D M
AU  - Defoe DM
FAU - Wondergem, R
AU  - Wondergem R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Membr Biol
JT  - The Journal of membrane biology
JID - 0211301
RN  - 0 (Marine Toxins)
RN  - 0 (Peptides)
RN  - 0 (Potassium Channels, Calcium-Activated)
RN  - 115422-61-2 (Charybdotoxin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 773HER9B6T (iberiotoxin)
RN  - G07GZ97H65 (Clotrimazole)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement/drug effects/*physiology
MH  - Charybdotoxin/pharmacology
MH  - Clotrimazole/pharmacology
MH  - Dogs
MH  - Epithelium/*drug effects/*physiology/ultrastructure
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Ion Channel Gating/drug effects/physiology
MH  - Kidney/drug effects/physiology/ultrastructure
MH  - Marine Toxins/pharmacology
MH  - Membrane Potentials/drug effects/physiology
MH  - Peptides/pharmacology
MH  - Potassium Channels, Calcium-Activated/*drug effects/*physiology
EDAT- 2003/01/18 04:00
MHDA- 2003/10/01 05:00
CRDT- 2003/01/18 04:00
PHST- 2002/04/19 00:00 [received]
PHST- 2003/01/18 04:00 [pubmed]
PHST- 2003/10/01 05:00 [medline]
PHST- 2003/01/18 04:00 [entrez]
AID - 10.1007/s00232-002-1045-1 [doi]
PST - ppublish
SO  - J Membr Biol. 2003 Jan 1;191(1):77-86. doi: 10.1007/s00232-002-1045-1.