PMID- 12532421
OWN - NLM
STAT- MEDLINE
DCOM- 20030325
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 104
IP  - 1
DP  - 2003 Mar 10
TI  - Molecular basis of basal cell carcinoma: analysis of differential gene expression 
      by differential display PCR and expression array.
PG  - 66-72
AB  - Basal cell carcinoma (BCC) is the most common tumor in the Caucasian population. 
      Although BCC rarely metastasize and cause death, they are problematic due to 
      their destructive growth and the frequent localization on the face. Until now the 
      knowledge of genes differentially expressed in BCC has been incomplete. To 
      elucidate the complex alterations in BCC-associated gene expression, we took 
      advantage of 2 techniques: the differential display RT-PCR (DD-PCR) and the 
      differential hybridization of cDNA arrays. Using DD-PCR, we showed differential 
      expression of genes known from other biological contexts (e.g., rac, ubiquitin 
      hydrolase), which could now be associated with BCC. In addition, we detected 
      unknown genes possibly contributing to the carcinogenesis of BCC. Of the 588 
      genes screened by differential hybridization of the Atlas human cDNA array, 
      differences in the expression levels of BCC were observed for 10 genes. These 
      data were obtained with RNA probes pooled from several BCC of different donors 
      and were subsequently confirmed by semiquantitative RT-PCR for Janus protein 
      tyrosine kinase 3 (Jak3), microsomal glutathione S-transferase 1 (GST 12), 
      teratocarcinoma-derived growth factor cripto, glutaredoxin and the monocyte 
      chemoattractant protein 1 (MCP-1) in 10 individual BCC specimens, 2 squamous cell 
      carcinoma (SCC), the cell line HaCaT and cultured normal human keratinocytes 
      (NHK) in comparison to normal skin. These genes are candidates from gene families 
      with known association to tumors, but they have not been reported in the 
      carcinogenesis of BCC yet. In summary, both approaches allow the detection of 
      differentially expressed genes possibly involved in the carcinogenesis of BCC.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Welss, Thomas
AU  - Welss T
AD  - Department of Dermatology, Heinrich-Heine University Dusseldorf, Dusseldorf, 
      Germany. Thomas.Welss@Henkel.com
FAU - Papoutsaki, Marina
AU  - Papoutsaki M
FAU - Michel, Gunter
AU  - Michel G
FAU - Reifenberger, Julia
AU  - Reifenberger J
FAU - Chimenti, Sergio
AU  - Chimenti S
FAU - Ruzicka, Thomas
AU  - Ruzicka T
FAU - Abts, Harry F
AU  - Abts HF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA, Complementary)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Carcinoma, Basal Cell/*genetics
MH  - Cell Line/metabolism
MH  - Cell Line, Transformed/metabolism
MH  - DNA, Complementary/genetics
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Library
MH  - Humans
MH  - Keratinocytes/metabolism
MH  - Neoplasm Proteins/biosynthesis/*genetics
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Messenger/biosynthesis/genetics
MH  - RNA, Neoplasm/biosynthesis/genetics
MH  - Skin Neoplasms/*genetics
MH  - Subtraction Technique
EDAT- 2003/01/18 04:00
MHDA- 2003/03/26 05:00
CRDT- 2003/01/18 04:00
PHST- 2003/01/18 04:00 [pubmed]
PHST- 2003/03/26 05:00 [medline]
PHST- 2003/01/18 04:00 [entrez]
AID - 10.1002/ijc.10912 [doi]
PST - ppublish
SO  - Int J Cancer. 2003 Mar 10;104(1):66-72. doi: 10.1002/ijc.10912.