PMID- 12533503
OWN - NLM
STAT- MEDLINE
DCOM- 20031126
LR  - 20131121
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 94
IP  - 5
DP  - 2003 May
TI  - Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma 
      cytokine levels after a 3-h run.
PG  - 1917-25
AB  - Sixteen experienced marathoners ran on treadmills for 3 h at approximately 70% 
      maximal oxygen consumption (Vo(2 max)) on two occasions while receiving 1 l/h 
      carbohydrate (CHO) or placebo (Pla) beverages. Blood and vastus lateralis muscle 
      biopsy samples were collected before and after exercise. Plasma was analyzed for 
      IL-6, IL-10, IL-1 receptor agonist (IL-1ra), IL-8, cortisol, glucose, and 
      insulin. Muscle was analyzed for glycogen content and relative gene expression of 
      13 cytokines by using real-time quantitative RT-PCR. Plasma glucose and insulin 
      were higher, and cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were 
      significantly lower postexercise in CHO vs. Pla. Change in muscle glycogen 
      content did not differ between CHO and Pla (P = 0.246). Muscle cytokine mRNA 
      content was detected preexercise for seven cytokines in this order (highest to 
      lowest): IL-15, TNF-alpha, IL-8, IL-1beta, IL-12p35, IL-6, and IFN-gamma. After 
      subjects ran for 3 h, gene expression above prerun levels was measured for five 
      of these cytokines: IL-1beta, IL-6, and IL-8 (large increases), and IL-10 and 
      TNF-alpha (small increases). The increase in mRNA (fold difference from 
      preexercise) was attenuated in CHO (15.9-fold) compared with Pla (35.2-fold) for 
      IL-6 (P = 0.071) and IL-8 (CHO, 7.8-fold; Pla, 23.3-fold; P = 0.063). CHO 
      compared with Pla beverage ingestion attenuates the increase in plasma IL-6, 
      IL-10, and IL-1ra and gene expression for IL-6 and IL-8 in athletes running 3 h 
      at 70% Vo(2 max) despite no differences in muscle glycogen content.
FAU - Nieman, D C
AU  - Nieman DC
AD  - Department of Health, Fischer Hamilton/Nycom Biochemistry Laboratory, Appalachian 
      State University, Boone, North Carolina 28608, USA. niemandc@appstate.edu
FAU - Davis, J M
AU  - Davis JM
FAU - Henson, D A
AU  - Henson DA
FAU - Walberg-Rankin, J
AU  - Walberg-Rankin J
FAU - Shute, M
AU  - Shute M
FAU - Dumke, C L
AU  - Dumke CL
FAU - Utter, A C
AU  - Utter AC
FAU - Vinci, D M
AU  - Vinci DM
FAU - Carson, J A
AU  - Carson JA
FAU - Brown, A
AU  - Brown A
FAU - Lee, W J
AU  - Lee WJ
FAU - McAnulty, S R
AU  - McAnulty SR
FAU - McAnulty, L S
AU  - McAnulty LS
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030117
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Blood Glucose)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Hormones)
RN  - 0 (Immunoglobulin A)
RN  - 0 (RNA, Messenger)
RN  - 9005-79-2 (Glycogen)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adult
MH  - Blood Cell Count
MH  - Blood Glucose/metabolism
MH  - Cytokines/*biosynthesis/*blood/genetics
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Dietary Carbohydrates/*pharmacology
MH  - Female
MH  - Gene Expression
MH  - Glycogen/metabolism
MH  - Hormones/blood
MH  - Humans
MH  - Hydrocortisone/blood
MH  - Immunoglobulin A/analysis/metabolism
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Oxygen Consumption/drug effects/physiology
MH  - RNA, Messenger/*biosynthesis/genetics
MH  - Running/*physiology
MH  - Saliva/chemistry/immunology
EDAT- 2003/01/21 04:00
MHDA- 2003/12/03 05:00
CRDT- 2003/01/21 04:00
PHST- 2003/01/21 04:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/01/21 04:00 [entrez]
AID - 01130.2002 [pii]
AID - 10.1152/japplphysiol.01130.2002 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2003 May;94(5):1917-25. doi: 
      10.1152/japplphysiol.01130.2002. Epub 2003 Jan 17.