PMID- 12535162
OWN - NLM
STAT- MEDLINE
DCOM- 20030326
LR  - 20190922
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 15
IP  - 2
DP  - 2003 Feb
TI  - Effect of intracerebroventricular administration of the octadecaneuropeptide on 
      the expression of pro-opiomelanocortin, neuropeptide Y and 
      corticotropin-releasing hormone mRNAs in rat hypothalamus.
PG  - 197-203
AB  - Intracerebroventricular (i.c.v.) administration of the octadecaneuropeptide 
      (diazepam-binding inhibitor [33-50]; ODN) exerts a potent anorexigenic effect in 
      the rat. We studied the effect of ODN on three neuropeptides involved in feeding 
      behaviour: the orexigenic peptide neuropeptide Y (NPY) and two anorexigenic 
      peptides, corticotropin-releasing hormone (CRH) and the pro-opiomelanocortin 
      (POMC)-derived peptide alpha-melanocyte-stimulating hormone. The effect of i.c.v. 
      administration of ODN (0.1 microg/kg and 1 microg/kg) on mRNA expression of the 
      peptides in male rat hypothalamus was evaluated by semiquantitative in situ 
      hybridization. In the arcuate nucleus, NPY-expressing neurones were mostly found 
      in the inner zone in close proximity of the third ventricle. ODN at the dose of 
      0.1 microg/kg induced a significant decrease of 17.4% in NPY mRNA expression, 
      while the depressing effect was more marked (31.4%) with the highest dose of ODN 
      (1 microg/kg). POMC-expressing neurones were more laterally located in the 
      arcuate nucleus. Administration of ODN at 0.1 microg/kg and 1 microg/kg doses 
      induced increases of 33.5% and 27.4% in POMC mRNA expression, respectively. 
      Labelling obtained with the CRH cRNA probe was essentially distributed throughout 
      the medial parvocellular area of the hypothalamic paraventricular nucleus. ODN, 
      at doses of 0.1 and 1 microg/kg, resulted in 17.8% and 32.8% decreases in CRH 
      mRNA expression, respectively. The present data suggest that ODN might exert its 
      anorexigenic effect by increasing mRNA expression of POMC and decreasing mRNA 
      expression of NPY in the arcuate nucleus.
FAU - Compere, V
AU  - Compere V
AD  - European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and 
      Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 
      Mont-Saint-Aignan, France.
FAU - Li, S
AU  - Li S
FAU - Leprince, J
AU  - Leprince J
FAU - Tonon, M C
AU  - Tonon MC
FAU - Vaudry, H
AU  - Vaudry H
FAU - Pelletier, G
AU  - Pelletier G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Appetite Depressants)
RN  - 0 (Diazepam Binding Inhibitor)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (diazepam binding inhibitor (33-50))
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Appetite Depressants/*pharmacology
MH  - Arcuate Nucleus of Hypothalamus/drug effects/physiology
MH  - Corticotropin-Releasing Hormone/*genetics
MH  - Diazepam Binding Inhibitor
MH  - Gene Expression/drug effects
MH  - Injections, Intraventricular
MH  - Male
MH  - Neuropeptide Y/*genetics
MH  - Neuropeptides/*pharmacology
MH  - Paraventricular Hypothalamic Nucleus/*drug effects/physiology
MH  - Peptide Fragments
MH  - Pro-Opiomelanocortin/*genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/01/22 04:00
MHDA- 2003/03/27 05:00
CRDT- 2003/01/22 04:00
PHST- 2003/01/22 04:00 [pubmed]
PHST- 2003/03/27 05:00 [medline]
PHST- 2003/01/22 04:00 [entrez]
AID - 970 [pii]
AID - 10.1046/j.1365-2826.2003.00970.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2003 Feb;15(2):197-203. doi: 10.1046/j.1365-2826.2003.00970.x.