PMID- 12536297
OWN - NLM
STAT- MEDLINE
DCOM- 20030422
LR  - 20151119
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 148
IP  - 1
DP  - 2003 Jan
TI  - Molecular diversity of RNA-2 genome segments in pecluviruses causing peanut clump 
      disease in West Africa and India.
PG  - 83-98
AB  - The complete nucleotide sequence of RNA-2 genome segments of four isolates of 
      Peanut clump virus (PCV) and two isolates of Indian peanut clump virus (IPCV) 
      were determined. Comparisons among the complete RNA-2 sequences of six isolates 
      from this study and two published earlier, revealed a high degree of variability 
      in size (between 4290 and 4652 nucleotides) and nucleotide sequence identities 
      (between 58 % and 79 %). Amino acid sequence alignments of the five open reading 
      frames (ORF) showed that ORF 4, which encodes the second of the triple gene block 
      proteins, is highly conserved (90 to 98 % identical) whereas the protein encoded 
      by ORF 2, whose function is unknown, is less conserved (25 to 60 % identical). 
      The coat protein of the eight isolates showed amino acid identities between 37 % 
      and 89 % and contained several conserved residues. Phylogenetic comparisons, 
      based on complete RNA-2 sequences, revealed that the eight isolates grouped into 
      two distinct clusters with no geographical distinction between PCV and IPCV 
      isolates. Phylogenetic tree topologies for individual ORFs showed an overall 
      similarity with that obtained from entire RNA-2 sequences, although the relative 
      positions of individual isolates vary within each cluster. The results indicate 
      that there is substantial divergence among the RNA-2 genomes of pecluviruses and 
      suggest that different proteins have evolved differently, possibly due to 
      different selection pressures.
FAU - Naidu, R A
AU  - Naidu RA
AD  - Department of Plant Pathology, University of Georgia, Athens, Georgia 30602, 
      U.S.A. naidu@arhes.uga.edu
FAU - Sawyer, S
AU  - Sawyer S
FAU - Deom, C M
AU  - Deom CM
LA  - eng
SI  - GENBANK/AF447396
SI  - GENBANK/AF447397
SI  - GENBANK/AF447398
SI  - GENBANK/AF447399
SI  - GENBANK/AF447400
SI  - GENBANK/AF447401
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Capsid Proteins)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Africa, Western
MH  - Amino Acid Sequence
MH  - Arachis/*virology
MH  - Base Sequence
MH  - Capsid/chemistry
MH  - Capsid Proteins/chemistry
MH  - Conserved Sequence
MH  - *Genes, Viral
MH  - India
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Plant Viruses/chemistry/classification/*genetics/isolation & purification
MH  - RNA Viruses/chemistry/classification/*genetics/isolation & purification
MH  - RNA, Viral/*genetics/isolation & purification
MH  - Sequence Alignment
EDAT- 2003/01/22 04:00
MHDA- 2003/04/23 05:00
CRDT- 2003/01/22 04:00
PHST- 2003/01/22 04:00 [pubmed]
PHST- 2003/04/23 05:00 [medline]
PHST- 2003/01/22 04:00 [entrez]
AID - 10.1007/s00705-002-0900-9 [doi]
PST - ppublish
SO  - Arch Virol. 2003 Jan;148(1):83-98. doi: 10.1007/s00705-002-0900-9.