PMID- 12536563
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20161020
IS  - 0257-7712 (Print)
IS  - 0257-7712 (Linking)
VI  - 32
IP  - 3
DP  - 2001 Sep
TI  - [Pilot study on mutations of p53 gene in laryngeal carcinoma].
PG  - 359-60, 434
AB  - OBJECTIVE: To determine the characteristic and frequency of the point mutation of 
      p53 gene. METHODS: 22 cases of laryngeal squamous cell carcinoma (LSCC) with 
      positive expression of mutant p53 protein and 2 cases of normal laryngeal 
      epithelium as control were examined by means of polymerase chain reaction 
      restriction fragment length polymorphism (PCR-RFLP), single strand conformation 
      polymorphism (SSCP) and DNA sequencing analyses. RESULTS: 3 cases of LSCC had the 
      point mutation on codons 248, 250 and 254 of exon 7 in p53 gene. None of the 
      cases had the point mutation on codon 249. CONCLUSION: These findings suggest 
      that the point mutation on codon 249 of exon 7 in p53 gene may be uncommon in 
      LSCC. SSCP and DNA sequencing analyses are sensitive and rapid methods for 
      detecting p53 gene mutation.
FAU - Zhou, G
AU  - Zhou G
AD  - Department of Otolaryngology, First Affiliated Hospital, WCUMS, Chengdu 610041, 
      China.
FAU - Zhang, X
AU  - Zhang X
FAU - Lui, S
AU  - Lui S
FAU - Lin, D
AU  - Lin D
FAU - Liang, C
AU  - Liang C
FAU - Yang, X
AU  - Yang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Hua Xi Yi Ke Da Xue Xue Bao
JT  - Hua xi yi ke da xue xue bao = Journal of West China University of Medical 
      Sciences = Huaxi yike daxue xuebao
JID - 8609552
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Exons
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Point Mutation
MH  - Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA
EDAT- 2003/01/23 04:00
MHDA- 2003/06/19 05:00
CRDT- 2003/01/23 04:00
PHST- 2003/01/23 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/01/23 04:00 [entrez]
PST - ppublish
SO  - Hua Xi Yi Ke Da Xue Xue Bao. 2001 Sep;32(3):359-60, 434.