PMID- 12536683
OWN - NLM
STAT- MEDLINE
DCOM- 20030505
LR  - 20131121
IS  - 1000-5625 (Print)
IS  - 1000-5625 (Linking)
VI  - 26
IP  - 3
DP  - 2001 Jun 28
TI  - [Growth inhibition of epidermal growth factor-stimulated human glioblastoma cells 
      by nicardipine in vito].
PG  - 211-4
AB  - OBJECTIVE: To study the growth effects of epidermal Growth Factor (EGF) and the 
      calcium channel antagonist nicardipine (NC) on U251 MG, a human malignant glioma 
      cell lime, which has high-affinity EGF receptors. METHODS: The growth effects of 
      EGF and NC on U251 MG cultured in serum-free and serum-containing (10% fetal 
      bovine serum, FBS) media respectively were observed by MTT colorimeritric 
      analysis. RESULTS: (1) EGF significantly enhanced the growth of U251 MG in a 
      dose-dependent manner in serum-free conditions. The near-maximal effect was 
      obtained at 10 mg.ml-1. The addition of serum obscured this effect. (2) NC 
      decreased U251 MG cells proliferation, especially in serum-containing media, and 
      completely blocked the growth-stimulated effect of EGF. The combinated effects of 
      EGF (10 ng.ml-1) and NC equaled those of NC alone. CONCLUSIONS: When serum is 
      absent U251 MG cells showed a pronounced mitogenic response to EGF in a 
      dosedependent manner, which approximated that achieved with 10% FBS. NC 
      suppressed the growth of U251 MG cells and completely blocked the 
      growth-stimulated effects of EGF, it can be used as an adjuvent therapy for human 
      malignant gliomas.
FAU - Huang, C X
AU  - Huang CX
AD  - Department of Neurosurgery, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Chen, B
AU  - Chen B
FAU - Zhang, Y F
AU  - Zhang YF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Hunan Yi Ke Da Xue Xue Bao
JT  - Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical 
      University
JID - 9424769
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Culture Media, Serum-Free)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - CZ5312222S (Nicardipine)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Brain Neoplasms/*pathology
MH  - Cell Division/drug effects
MH  - Culture Media, Serum-Free
MH  - Epidermal Growth Factor/*pharmacology
MH  - Glioblastoma/*pathology
MH  - Humans
MH  - Nicardipine/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2003/01/23 04:00
MHDA- 2003/05/06 05:00
CRDT- 2003/01/23 04:00
PHST- 2003/01/23 04:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2003/01/23 04:00 [entrez]
PST - ppublish
SO  - Hunan Yi Ke Da Xue Xue Bao. 2001 Jun 28;26(3):211-4.