PMID- 12536722
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20061115
IS  - 1000-5625 (Print)
IS  - 1000-5625 (Linking)
VI  - 26
IP  - 4
DP  - 2001 Aug 28
TI  - [Protective effect of ginsenosides on human peritoneal mesothelial cell against 
      lactate-based peritoneal dialysis solutions--induced injury in vitro].
PG  - 317-20
AB  - OBJECTIVE: The aim of this research was to observe the influence of ginsenosides 
      (GS) on viability and proliferation of human peritoneal mesothelial cell (HPMC) 
      which were inhibited by L-PDS in vitro. METHOD: Mesothelial cells were isolated 
      from human omental specimens by trypsin disaggregation and established a stable 
      HPMC culture model. The viability and capacity of proliferation of HPMC were 
      assessed by lactate dehydrognase (LDH) release and tetrazolium salt colorimetry 
      assay (MTT assay). RESULTS: Compared with the control group, the release of LDH 
      in L-PDS groups (2.5%, 4.25% glucose) significantly increased. But there was no 
      difference between the two glucose concentrations. As HPMC pre-exposed to L-PDS 
      prolonged, the viability of cells decreased gradually. GS decreased the release 
      of LDH and increased the cells viability (P < 0.01), as well as enhanced the 
      proliferation of HPMC. CONCLUSION: The results suggest that GS has some 
      protective effects on cell viability and proliferation of HPMC inhibited by L-PDS 
      in vitro.
FAU - Ye, Y
AU  - Ye Y
AD  - Department of Geriatrics, Second Xiangya Hospital, Central South University, 
      Changsha 410011, China.
FAU - Peng, Y M
AU  - Peng YM
FAU - Liu, F Y
AU  - Liu FY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Hunan Yi Ke Da Xue Xue Bao
JT  - Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical 
      University
JID - 9424769
RN  - 0 (Dialysis Solutions)
RN  - 0 (Ginsenosides)
RN  - 0 (Lactates)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dialysis Solutions/*adverse effects
MH  - Epithelium
MH  - Ginsenosides/*pharmacology
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lactates/adverse effects
MH  - Omentum/cytology
MH  - Peritoneum/*cytology
EDAT- 2003/01/23 04:00
MHDA- 2003/06/19 05:00
CRDT- 2003/01/23 04:00
PHST- 2003/01/23 04:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/01/23 04:00 [entrez]
PST - ppublish
SO  - Hunan Yi Ke Da Xue Xue Bao. 2001 Aug 28;26(4):317-20.