PMID- 12537978
OWN - NLM
STAT- MEDLINE
DCOM- 20030425
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 27
IP  - 3
DP  - 2003 Mar
TI  - The in vitro generation of Ph1+ ALL-specific HLA-A24-restricted cytotoxic T 
      lymphocytes using a synthetic 16 mer minor bcr-abl peptide.
PG  - 253-7
AB  - Sixteen mer peptide, which spans the junctional region of the acute lymphoid 
      leukemia (ALL)-specific minor bcr-abl fusion protein and contains a motif that 
      can bind to human leukocyte antigen (HLA)-A24, was constructed. We tried to 
      generate Philadelphia chromosome 1 (Ph1) positive ALL-specific cytotoxic T 
      lymphocytes (CTLs) from eight normal HLA-A24+ individuals with peptide-pulsed 
      autologous dendritic cells (DCs). CTLs could be generated from the mononuclear 
      cells (MNCs) of a single donor, which could kill peptide-pulsed autologous DCs 
      and two A24+ ALL lines, while an HLA-A24+ CML line was only weakly killed and 
      unpulsed DCs or the control lines Daudi or K562 were not recognized. Those CTLs 
      consisted predominantly of CD8+ T cells whose cytotoxicity could be neutralized 
      by monoclonal antibodies to HLA-class I or HLA-A24, and also produced interferon 
      (IFN)-gamma after being stimulated with peptide-pulsed DCs.
FAU - Hagihara, Masao
AU  - Hagihara M
AD  - Department of Hematology and Rheumatology, Tokai University School of Medicine, 
      Bohseidai, Isehara, Kanagawa 259-1193, Japan. masaoha@is.icc.u-tokai.ac.jp
FAU - Tsuchiya, Takahide
AU  - Tsuchiya T
FAU - Hyodo, Osamu
AU  - Hyodo O
FAU - Ueda, Yoko
AU  - Ueda Y
FAU - Tazume, Kei
AU  - Tazume K
FAU - Gansuvd, Balgansuren
AU  - Gansuvd B
FAU - Kato, Shunichi
AU  - Kato S
FAU - Hotta, Tomomitsu
AU  - Hotta T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Oligopeptides)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Coculture Techniques
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/immunology
MH  - Fusion Proteins, bcr-abl/*immunology
MH  - HLA-A Antigens/*immunology
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Killer Cells, Lymphokine-Activated/immunology
MH  - Oligopeptides/chemical synthesis/*immunology
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*immunology/pathology
MH  - T-Lymphocytes, Cytotoxic/cytology/*immunology
MH  - Tumor Cells, Cultured
EDAT- 2003/01/23 04:00
MHDA- 2003/04/26 05:00
CRDT- 2003/01/23 04:00
PHST- 2003/01/23 04:00 [pubmed]
PHST- 2003/04/26 05:00 [medline]
PHST- 2003/01/23 04:00 [entrez]
AID - S0145212602000929 [pii]
AID - 10.1016/s0145-2126(02)00092-9 [doi]
PST - ppublish
SO  - Leuk Res. 2003 Mar;27(3):253-7. doi: 10.1016/s0145-2126(02)00092-9.