PMID- 12539387
OWN - NLM
STAT- MEDLINE
DCOM- 20030418
LR  - 20100429
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 17
IP  - 4
DP  - 1999 Nov
TI  - [Effects of growth factors on DNA and collagen synthesis of human mandibular 
      condylar cartilage cells].
PG  - 358-60
AB  - OBJECTIVE: To investigate the effects of transforming growth factor-beta 1 
      (TGF-beta 1), insulin-like growth factor-I (IGF-I) and basic fibroblast growth 
      factor(bFGF) on DNA and collagen synthesis of mandibular condylar cartilage(MCC) 
      of human fetus. METHODS: Cell culture, 3H-TdR and 3H-Proline incorporation 
      methods were used. MCC cells were harvested from 4 to 5 months old human fetus. 
      Cells were seeded at 2 x 10(4)/well on 96-well Plate. After synchronization, 
      medium was replaced by DMEM containing 0.4% NCS with various growth factors and 
      concentrations. RESULTS: bFGF stimulated the DNA synthesis significantly, and 
      IGF-I had less effect, while the effect of TGF-beta 1 was insignificant. For 
      collagen synthesis, bFGF caused a dose-dependent increase(60%). A greater 
      effect(98%) was achieved when IGF-1 was added. In contrast, TGF-beta 1 could 
      inhibit collagen synthesis (24%). CONCLUSION: Growth factors play an important 
      part in the proliferation and matrix synthesis of MCC cells, which might be of 
      potential application in treating cartilage destructive lesions.
FAU - Jiao, Y
AU  - Jiao Y
AD  - College of Stomatology, West China University of Medical Sciences.
FAU - Wang, D
AU  - Wang D
FAU - Han, W
AU  - Han W
FAU - Li, T
AU  - Li T
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Collagen Type II)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Chondrocytes/*drug effects/metabolism
MH  - Collagen Type II/*biosynthesis
MH  - DNA/*biosynthesis
MH  - Fibroblast Growth Factors/pharmacology
MH  - Humans
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Mandibular Condyle/*cytology
MH  - Transforming Growth Factor beta/pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2003/01/24 04:00
MHDA- 2003/04/19 05:00
CRDT- 2003/01/24 04:00
PHST- 2003/01/24 04:00 [pubmed]
PHST- 2003/04/19 05:00 [medline]
PHST- 2003/01/24 04:00 [entrez]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 1999 Nov;17(4):358-60.