PMID- 12542090
OWN - NLM
STAT- MEDLINE
DCOM- 20030318
LR  - 20061115
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 52
IP  - 2
DP  - 2002 Nov 22
TI  - Dormancy as a survival strategy of the fish pathogen Streptococcus parauberis in 
      the marine environment.
PG  - 129-36
AB  - The fate of Streptococcus parauberis in seawater and sediment microcosms at 
      different temperatures (6 and 22 degrees C) was investigated by comparing the 
      survival dynamics of 2 strains of this bacterial species, isolated respectively 
      from diseased turbot and cattle. The turbot and the bovine isolate showed similar 
      survival kinetics, remaining culturable for approximately 1 mo in water and 6 mo 
      in sediment. A slight influence of temperature on the stability of the cells was 
      observed, in that the number of culturable cells was about 1 log10 unit higher at 
      6 than at 22 degrees C. During the starvation period, the metabolic activity of 
      the cells, after suffering a strong reduction during the first 12 d, stabilized 
      at levels ranging from 20 to 40% of the initial values. However, in all the 
      microcosms, the acridine orange (AO) and 4',6-diamidino-2-phenilindole (DAPI) 
      counts remained at about 10(5) cells ml(-1) throughout the experimental period, 
      even when cells became undetectable by standard plate count methods. The addition 
      of fresh medium to microcosms containing nonculturable cells induced the return 
      to culturability of S. parauberis strains. On the basis of these results, it 
      seems that S. parauberis has the ability to enter into a viable but nonculturable 
      (VBNC) state. Dormant cells of the turbot isolate maintained their infectivity 
      and pathogenic potential for fish.
FAU - Curras, M
AU  - Curras M
AD  - Departamento de Microbiologia y Parasitologia, Facultad de Biologia, Universidad 
      de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
FAU - Magarinos, B
AU  - Magarinos B
FAU - Toranzo, A E
AU  - Toranzo AE
FAU - Romalde, J L
AU  - Romalde JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Colony Count, Microbial/veterinary
MH  - Disease Reservoirs/*veterinary
MH  - Fish Diseases/etiology/*microbiology
MH  - Flatfishes/*microbiology
MH  - Geologic Sediments/microbiology
MH  - Kinetics
MH  - Seawater/microbiology
MH  - Streptococcus/growth & development/isolation & 
      purification/*pathogenicity/*physiology
MH  - Temperature
MH  - Time Factors
MH  - Virulence/physiology
MH  - *Water Microbiology
EDAT- 2003/01/25 04:00
MHDA- 2003/03/19 04:00
CRDT- 2003/01/25 04:00
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2003/03/19 04:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 10.3354/dao052129 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2002 Nov 22;52(2):129-36. doi: 10.3354/dao052129.