PMID- 12542639
OWN - NLM
STAT- MEDLINE
DCOM- 20030403
LR  - 20191106
IS  - 0962-1075 (Print)
IS  - 0962-1075 (Linking)
VI  - 12
IP  - 1
DP  - 2003 Feb
TI  - Application of 16S rDNA-DGGE to examine the microbial ecology associated with a 
      social wasp Vespula germanica.
PG  - 85-91
AB  - Invertebrates host numerous bacteria, with interactions ranging from pathogenesis 
      to symbiosis. While certain symbiotic relationships have been well studied, 
      little is known about the dynamics of these bacterial communities. Denaturing 
      gradient gel electrophoresis (DGGE) was used to examine the bacterial microflora 
      associated with the eusocial wasp Vespula germanica. DGGE profiles of larval guts 
      revealed a variable microflora, suggesting that V. germanica is not dependent on 
      a particular suite of mutualists. The variation in profiles was not related to 
      season, nest size or macrohabitat. Sequences corresponding to Lactococcus, 
      Lactobacillus, a novel Leuconostoc and two Rickettsiella grylli strains were 
      obtained. DGGE proved to be a useful technique for characterizing the wasp 
      microflora. Given the importance of microbial communities to invertebrates, there 
      is much to be gained from the application of such techniques.
FAU - Reeson, A F
AU  - Reeson AF
AD  - Applied & Molecular Ecology, Adelaide University, 5005, South Australia. 
      andrew.reeson@dpi.qld.gov.au
FAU - Jankovic, T
AU  - Jankovic T
FAU - Kasper, M L
AU  - Kasper ML
FAU - Rogers, S
AU  - Rogers S
FAU - Austin, A D
AU  - Austin AD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Insect Mol Biol
JT  - Insect molecular biology
JID - 9303579
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/chemistry/genetics
MH  - Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Lactobacillus/genetics/isolation & purification
MH  - Lactococcus/genetics/isolation & purification
MH  - Leuconostoc/genetics/isolation & purification
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Rickettsia/genetics/isolation & purification
MH  - Sequence Analysis, DNA
MH  - South Australia
MH  - Wasps/*microbiology
EDAT- 2003/01/25 04:00
MHDA- 2003/04/04 05:00
CRDT- 2003/01/25 04:00
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2003/04/04 05:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 390 [pii]
AID - 10.1046/j.1365-2583.2003.00390.x [doi]
PST - ppublish
SO  - Insect Mol Biol. 2003 Feb;12(1):85-91. doi: 10.1046/j.1365-2583.2003.00390.x.