PMID- 12542936
OWN - NLM
STAT- MEDLINE
DCOM- 20050302
LR  - 20171116
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 8
IP  - 6
DP  - 2002 Dec
TI  - Replicative aging and gene expression in long-term cultures of human bone marrow 
      stromal cells.
PG  - 901-10
AB  - Bone marrow stromal cells (BMSCs) can be easily isolated from adult marrow and 
      contain a population of pluripotent progenitors that can give rise to different 
      mesenchymal lineages both in vitro and in vivo. These properties make BMSCs an 
      attractive target for cell-based therapeutic strategies for a variety of 
      disorders. However, because of their low frequency in vivo, to obtain a 
      sufficient number of cells for tissue engineering a step of extensive in vitro 
      expansion is required, which could significantly alter BMSC properties. 
      Therefore, effective therapeutic use of BMSCs requires the design of appropriate 
      approaches for in vitro cell expansion. In this study we have investigated the 
      biological effects of in vitro expansion on BMSC proliferative ability and on 
      their spontaneous differentiation. Telomerase activity and telomere shortening 
      kinetics were evaluated together with variations in osteogenic, chondrogenic, and 
      adipogenic gene expression throughout the BMSC life span. In culture BMSCs never 
      displayed telomerase activity and during in vitro expansion telomere length 
      decreased. Furthermore, gene expression patterns spontaneously varied during 
      expansion, indicating a progressive commitment of the population toward the 
      osteogenic lineage. In conclusion, BMSCs in culture undergo progressive 
      replicative aging and osteogenic differentiation. These observations are relevant 
      to their successful use in clinics and should be considered when designing novel 
      therapeutic strategies.
FAU - Banfi, Andrea
AU  - Banfi A
AD  - Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
FAU - Bianchi, Giordano
AU  - Bianchi G
FAU - Notaro, Rosario
AU  - Notaro R
FAU - Luzzatto, Lucio
AU  - Luzzatto L
FAU - Cancedda, Ranieri
AU  - Cancedda R
FAU - Quarto, Rodolfo
AU  - Quarto R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cell Culture Techniques
MH  - Cell Division/physiology
MH  - Cellular Senescence/*physiology
MH  - Child
MH  - Child, Preschool
MH  - Gene Expression/*physiology
MH  - Humans
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/cytology/physiology
MH  - Telomerase/metabolism
EDAT- 2003/01/25 04:00
MHDA- 2005/03/03 09:00
CRDT- 2003/01/25 04:00
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2005/03/03 09:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 10.1089/107632702320934001 [doi]
PST - ppublish
SO  - Tissue Eng. 2002 Dec;8(6):901-10. doi: 10.1089/107632702320934001.