PMID- 12544823
OWN - NLM
STAT- MEDLINE
DCOM- 20030425
LR  - 20231213
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 14
IP  - 1
DP  - 2003 Jan 20
TI  - Involvement of CLOCK:BMAL1 heterodimer in serum-responsive mPer1 induction.
PG  - 15-9
AB  - A rapid induction of mouse period1 (mPer1) gene expression is supposed to be 
      critical in the clock gene regulation, especially in the phase resetting of the 
      clock, but its molecular mechanism is poorly understood. Based on the previous 
      finding that the process does not involve de novo synthesis of proteins, we 
      postulated the involvement of CLOCK:BMAL1 heterodimer, a positive regulator of 
      circadian oscillator, in the rapid induction of mPer1 transcription. To test this 
      hypothesis, we utilized CLOCKdelta19, a dominant-negative mutant, to suppress the 
      function of CLOCK:BMAL1 in vitro. Serum-evoked rapid increases of mPer1 mRNA 
      expression and promoter activity were significantly blunted when CLOCK:BMAL1 
      function was interfered with. Furthermore, DNA binding activity of CLOCK:BMAL1 
      heterodimer to five E-boxes of mPer1 promoter markedly increased shortly after 
      serum shock. Taken together, these results suggest that CLOCK:BMAL1 heterodimer 
      is not only a core component of negative feedback loop driving circadian 
      oscillator, but also involved in the rapid induction of mPer1during phase 
      resetting of the clock.
CI  - Copyright 2003 Lippincott Williams & Wilkins
FAU - Jung, Hosung
AU  - Jung H
AD  - Development and Neuroendocrine Research Laboratory, School of Biomedical 
      Sciences, Seoul National University, Korea.
FAU - Choe, Youngshik
AU  - Choe Y
FAU - Kim, Hyunjung
AU  - Kim H
FAU - Park, Noheon
AU  - Park N
FAU - Son, Gi Hoon
AU  - Son GH
FAU - Khang, Inkoo
AU  - Khang I
FAU - Kim, Kyungjin
AU  - Kim K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (ARNTL Transcription Factors)
RN  - 0 (Bmal1 protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Per1 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.3.1.48 (CLOCK Proteins)
RN  - EC 2.3.1.48 (Clock protein, mouse)
SB  - IM
MH  - 3T3 Cells/drug effects
MH  - ARNTL Transcription Factors
MH  - Alleles
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - CLOCK Proteins
MH  - Cattle
MH  - Cell Cycle Proteins
MH  - Circadian Rhythm/*physiology
MH  - Culture Media, Serum-Free/pharmacology
MH  - DNA/genetics/metabolism
MH  - Dimerization
MH  - Feedback, Physiological
MH  - Fetal Blood/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Genes, Dominant
MH  - Genes, Reporter
MH  - Genes, fos
MH  - Luciferases/biosynthesis/genetics
MH  - Mice
MH  - Nuclear Proteins/*biosynthesis/genetics
MH  - Period Circadian Proteins
MH  - Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-fos/biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Recombinant Fusion Proteins/physiology
MH  - Trans-Activators/chemistry/genetics/*physiology
MH  - Transcription Factors/chemistry/*physiology
MH  - Transfection
EDAT- 2003/01/25 04:00
MHDA- 2003/04/26 05:00
CRDT- 2003/01/25 04:00
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2003/04/26 05:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 10.1097/00001756-200301200-00003 [doi]
PST - ppublish
SO  - Neuroreport. 2003 Jan 20;14(1):15-9. doi: 10.1097/00001756-200301200-00003.