PMID- 12544869
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20220317
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 75
IP  - 1
DP  - 2003 Jan 15
TI  - Single human leukocyte antigen flow cytometry beads for accurate identification 
      of human leukocyte antigen antibody specificities.
PG  - 43-9
AB  - BACKGROUND: It is difficult to assign antibody specificity for highly sensitized 
      patients using a cell panel with multiple antigens per reaction. We describe here 
      a single antigen bead panel for accurate identification of human leukocyte 
      antigen (HLA) antibody specificities by flow cytometry. METHODS: A total of 110 
      single recombinant HLAs, including 34 A locus alleles, 57 B locus alleles, and 19 
      C locus alleles, were produced by a mammalian expression system. These single 
      antigens were coated onto eight different colored microbeads, which were mixed 
      together in one tube for simultaneous detection of HLA antibodies against eight 
      different antigens per flow cytometry test. RESULTS: Single HLA reacted 
      specifically with the serologically defined monoclonal antibodies. The single 
      antigen panel provided higher resolution than the regular cell panel for antibody 
      detection by uncovering the masked specificities. Single antigens also provided 
      higher sensitivity than the multiple antigens coated onto beads for HLA antibody 
      detection as demonstrated by serum dilution studies. In 10 sera from patients who 
      had rejected a kidney transplant, single antigen beads identified antibodies to 
      31 of 35 antigens that were mismatched in the donor. Most important, none of the 
      reactions were against antigens present in the recipient. CONCLUSION: An accurate 
      and sensitive HLA antibody detection method is described using flow cytometry 
      beads coated with single HLAs produced by recombinant technology. The single 
      antigen beads should be useful in predicting negative crossmatch in highly 
      sensitized organ recipients and highly sensitized patients requiring platelets.
FAU - Pei, Rui
AU  - Pei R
AD  - Research Department, One Lambda. Inc., Canoga Park, CA, USA. rpei@onelambda.com
FAU - Lee, Jar-How
AU  - Lee JH
FAU - Shih, Neng-Jen
AU  - Shih NJ
FAU - Chen, Mike
AU  - Chen M
FAU - Terasaki, Paul I
AU  - Terasaki PI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Isoantibodies)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - *Antibody Specificity
MH  - Flow Cytometry/*methods
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Isoantibodies/*blood/immunology
MH  - Recombinant Proteins/immunology
EDAT- 2003/01/25 04:00
MHDA- 2003/02/07 04:00
CRDT- 2003/01/25 04:00
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 10.1097/00007890-200301150-00008 [doi]
PST - ppublish
SO  - Transplantation. 2003 Jan 15;75(1):43-9. doi: 10.1097/00007890-200301150-00008.