PMID- 12545335
OWN - NLM
STAT- MEDLINE
DCOM- 20030805
LR  - 20081121
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 16
IP  - 1
DP  - 2003 Jan
TI  - Differential intragraft cytokine messenger RNA profiles during rejection and 
      repair of clinical heart transplants. A longitudinal study.
PG  - 9-14
AB  - After clinical heart transplantation, ischemia, acute rejection, and repair 
      mechanisms can trigger the up-regulation of cytokines. To investigate the 
      cytokine profile early after transplantation, we monitored messenger RNA (mRNA) 
      expression levels of tumor necrosis factor-alpha (TNF-alpha), monocyte 
      chemoattractant protein-1 (MCP-1), transforming growth factor-beta (TGF-beta), 
      platelet-derived growth factor-A (PDGF-A), and basic fibroblast growth factor 
      (bFGF) by reverse transcriptase-polymerase chain reaction (RT-PCR) in serial 
      endomyocardial biopsies ( n=123) from 16 cardiac allograft recipients during the 
      first 3 post-operative months. In the first month, mRNA expression levels of 
      MCP-1, TNF-alpha, TGF-beta, and bFGF were significantly higher than in the period 
      thereafter (acute rejection episodes excluded). Acute rejection (International 
      Society for Heart and Lung Transplantation (ISHLT) rejection grade >2) was 
      strongly associated with the level of TNF-alpha mRNA. After acute rejection 
      episodes, rising mRNA expression levels of PDGF-A and bFGF were found. The 
      association between TNF-alpha mRNA and acute rejection reflects the importance of 
      this cytokine in allogeneic responses. Elevated growth factor expression levels 
      indicate repair responses after tissue damage due to either the transplantation 
      procedure (surgery, ischemia, reperfusion) or acute allograft rejection.
FAU - de Groot-Kruseman, Hester A
AU  - de Groot-Kruseman HA
AD  - Department of Internal Medicine, Erasmus MC, PO Box 1738, 3000 DR Rotterdam, The 
      Netherlands.
FAU - Mol, Wendy M
AU  - Mol WM
FAU - Niesters, Hubert G M
AU  - Niesters HG
FAU - Maat, Alex P W
AU  - Maat AP
FAU - van Gelder, Teun
AU  - van Gelder T
FAU - Balk, Aggie H M M
AU  - Balk AH
FAU - Weimar, Willem
AU  - Weimar W
FAU - Baan, Carla C
AU  - Baan CC
LA  - eng
PT  - Journal Article
DEP - 20021210
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (platelet-derived growth factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Acute Disease
MH  - Biopsy
MH  - Chemokine CCL2/genetics
MH  - Cold Temperature
MH  - Fibroblast Growth Factor 2/genetics
MH  - Gene Expression/immunology
MH  - Graft Rejection/immunology/pathology/*physiopathology
MH  - *Heart Transplantation
MH  - Humans
MH  - Longitudinal Studies
MH  - Myocardial Ischemia/physiopathology
MH  - Myocardium/pathology
MH  - Platelet-Derived Growth Factor/genetics
MH  - RNA, Messenger/analysis
MH  - Transforming Growth Factor beta/genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2003/01/25 04:00
MHDA- 2003/08/06 05:00
CRDT- 2003/01/25 04:00
PHST- 2001/10/08 00:00 [received]
PHST- 2002/08/12 00:00 [revised]
PHST- 2002/08/22 00:00 [accepted]
PHST- 2003/01/25 04:00 [pubmed]
PHST- 2003/08/06 05:00 [medline]
PHST- 2003/01/25 04:00 [entrez]
AID - 10.1007/s00147-002-0489-7 [doi]
PST - ppublish
SO  - Transpl Int. 2003 Jan;16(1):9-14. doi: 10.1007/s00147-002-0489-7. Epub 2002 Dec 
      10.