PMID- 12545812
OWN - NLM
STAT- MEDLINE
DCOM- 20030428
LR  - 20161020
IS  - 0257-7712 (Print)
IS  - 0257-7712 (Linking)
VI  - 31
IP  - 3
DP  - 2000 Sep
TI  - [Experimental studies on exons 5-8 of p53 gene mutation in laryngeal squamous 
      cell carcinoma].
PG  - 295-7
AB  - This study was designed to detect the point mutations of exons 5-8 of p53 gene in 
      laryngeal squamous cell carcinoma (LSCC) and analyze their relationship. The 
      detection of fresh tumor samples from LSCC patients was performed using silver 
      staining PCR-SSCP method. From among 60 patients samples, 47 were positive in 
      SSCP. Mutation rate was 78.3% (47/60). The results showed that the prevalence of 
      p53 mutations in LSCC subjected to silver staining PCR-SSCP test were 50% (30/60) 
      in exon 5, 11.67%(7/60) in exon 6, 41.6%(25/60) in exon 7, and 25%(15/60) in exon 
      8. The majority of the mutations were found in exon 5 and exon 7. Exon 5 and exon 
      7 of p53 gene may be the mutation hotspot in LSCC; they may be the critical 
      position easily attacked by some carcinogen factors relating to LSCC.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Otorhinolaryngology, Fourth Affiliated Hospital, WCUMS, Chengdu 
      610041.
FAU - Liu, S
AU  - Liu S
FAU - Zhou, G
AU  - Zhou G
FAU - Liang, C
AU  - Liang C
FAU - Lin, D
AU  - Lin D
FAU - Qiao, X
AU  - Qiao X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Hua Xi Yi Ke Da Xue Xue Bao
JT  - Hua xi yi ke da xue xue bao = Journal of West China University of Medical 
      Sciences = Huaxi yike daxue xuebao
JID - 8609552
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Exons
MH  - Genes, p53/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - *Point Mutation
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Single-Stranded Conformational
MH  - Tumor Suppressor Protein p53/genetics
EDAT- 2003/01/28 04:00
MHDA- 2003/04/29 05:00
CRDT- 2003/01/28 04:00
PHST- 2003/01/28 04:00 [pubmed]
PHST- 2003/04/29 05:00 [medline]
PHST- 2003/01/28 04:00 [entrez]
PST - ppublish
SO  - Hua Xi Yi Ke Da Xue Xue Bao. 2000 Sep;31(3):295-7.