PMID- 12547157
OWN - NLM
STAT- MEDLINE
DCOM- 20030212
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 139
IP  - 1
DP  - 2002 Nov
TI  - Coamplification of 12p11 and 12q13 approximately q22 in multiple ring chromosomes 
      in a spindle cell sarcoma resolved by novel multicolor fluorescence in situ 
      hybridization analysis.
PG  - 44-7
AB  - An 80-year-old male presented with a lobulated mass in the lower abdominal wall. 
      A diagnosis of an intermediate grade myofibroblastic spindle cell sarcoma was 
      made. Cytogenetic analysis demonstrated a complex karyotype with a der(6), a 
      small marker and five, different in size, ring chromosomes. Fluorescence in situ 
      hybridization (FISH), multiplex FISH, and multicolor banding analysis was used to 
      further delineate this complex karyotype. The der(6) was shown to be a 
      der(18)t(6;18;9;12;18), the marker chromosome was identified as del(17), and the 
      ring chromosomes as r(9) and r(12;18)x4. Amplification of 18 and coamplification 
      of 12p and 12q was detected in the ring and marker chromosomes. No intercellular 
      heterogeneity was observed although a few micronuclei containing chromosome 18 
      and anaphase bridges, containing chromosome 12 material, the result of 
      bridge-fusion-bridge (BFB) cycles, were observed. Our findings combined with 
      results from others indicate that amplification of chromosomes 12 and 18 as well 
      as BFB phenomena characterize this type of sarcoma.
FAU - Lestou, Valia S
AU  - Lestou VS
AD  - Department of Pathology and Laboratory Medicine, British Columbia Cancer Agency, 
      r, British Columbia, Vancouve, Canada. vlestou@bccancer.bc.ca
FAU - O'Connell, John X
AU  - O'Connell JX
FAU - Ludkovski, Olga
AU  - Ludkovski O
FAU - Gosling, Helena
AU  - Gosling H
FAU - Lesack, David
AU  - Lesack D
FAU - Horsman, Douglas E
AU  - Horsman DE
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 9
MH  - Gene Amplification/genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - *Ring Chromosomes
MH  - Sarcoma/*genetics
EDAT- 2003/01/28 04:00
MHDA- 2003/02/14 04:00
CRDT- 2003/01/28 04:00
PHST- 2003/01/28 04:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2003/01/28 04:00 [entrez]
AID - S0165460802006003 [pii]
AID - 10.1016/s0165-4608(02)00600-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Nov;139(1):44-7. doi: 10.1016/s0165-4608(02)00600-3.