PMID- 12548612
OWN - NLM
STAT- MEDLINE
DCOM- 20030211
LR  - 20181130
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 97
IP  - 3
DP  - 2003 Feb 1
TI  - Detection of chromosomal aneusomy by fluorescence in situ hybridization for 
      patients with nipple discharge.
PG  - 690-4
AB  - BACKGROUND: Breast carcinoma and precancer are believed to start in the lining of 
      the milk duct or lobule. Ductography and fiberoptic ductoscopy (FDS) are used to 
      identify abnormal intraductal lesions, although it is difficult to distinguish 
      malignant from benign cases. Therefore, we studied the clinical usefulness of 
      fluorescence in situ hybridization (FISH) analysis of a numerical aberration of 
      chromosomes (aneusomy) using ductal lavage from patients with nipple discharge. 
      METHODS: We applied ductography and FDS to 90 women who had nipple discharge. 
      Ductal lavages obtained from patients with positive ductography and/or FDS 
      findings were subjected to cytology and FISH analysis using centromere probes for 
      chromosomes 1, 11, and 17. Patients with samples that showed aneusomy in at least 
      one of the three chromosomes were diagnosed as positive. RESULTS: Histologic 
      evaluation revealed 54 benign lesions and six malignancies. The sensitivity, 
      specificity, and diagnostic accuracy were 33.3%, 88.9%, and 83.3%, respectively, 
      for cytology and 100%, 100%, and 100%, respectively, for FISH. CONCLUSION: The 
      results demonstrated that FISH has a diagnostic accuracy comparable to cytology. 
      This technique has 100% specificity is making a definitive diagnosis of 
      malignancy in patients with indeterminate cytologic results, suggesting that FISH 
      diagnosis can be a good adjunct to cytology.
CI  - Copyright 2003 American Cancer Society.DOI 10.1002/cncr.11091
FAU - Yamamoto, Daigo
AU  - Yamamoto D
AD  - Department of Surgery, Kansai Medical University, Osaka, Japan. 
      yamamotd@takii.kmu.ac.jp
FAU - Senzaki, Hideto
AU  - Senzaki H
FAU - Nakagawa, Hiroyuki
AU  - Nakagawa H
FAU - Okugawa, Homa
AU  - Okugawa H
FAU - Gondo, Hideki
AU  - Gondo H
FAU - Tanaka, Kanji
AU  - Tanaka K
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*diagnosis/diagnostic imaging/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Cytodiagnosis/*methods
MH  - Endoscopy
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Nipples/*metabolism
MH  - Radiography
MH  - Sensitivity and Specificity
MH  - Therapeutic Irrigation
EDAT- 2003/01/28 04:00
MHDA- 2003/02/13 04:00
CRDT- 2003/01/28 04:00
PHST- 2003/01/28 04:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2003/01/28 04:00 [entrez]
AID - 10.1002/cncr.11091 [doi]
PST - ppublish
SO  - Cancer. 2003 Feb 1;97(3):690-4. doi: 10.1002/cncr.11091.