PMID- 12549216
OWN - NLM
STAT- MEDLINE
DCOM- 20030506
LR  - 20151119
IS  - 0001-5334 (Print)
IS  - 0001-5334 (Linking)
VI  - 34
IP  - 3
DP  - 2001 Sep
TI  - [Detection and analysis of alien genes in transgenic rice by fluorescence in situ 
      hybridization].
PG  - 163-8
AB  - Using fluorescence in situ hybridization (FISH) with metaphase preparations, we 
      localized a transferred barnase-psl DNA sequence onto chromosomes in 8 rice 
      transgenic plants. All the tested rice transgenic lines showed hybridization 
      signals on the middle and terminal regions of chromosome arms except for those 
      close to centromeres. In two lines, two different integration sites were 
      identified, and the other lines showed only one integration site. With the aid of 
      Southern analysis and expression detection, we found that the barnase tended to 
      show a higher level expression in the lines whose integration sites near the 
      distal regions of chromosomes, while the expression level became lower in the 
      lines whose integration sites near the centromeres. This result suggested a 
      possible relationship between chromosomal location of transgenes and the 
      expression level. However it showed no obvious relationship between copy numbers 
      and expression levels. In most cases, the results of multi-color FISH showed that 
      barnase-ps1 always integrated at the same position on the chrmosome as the 
      reporter genes(pHctinG).
FAU - Jin, W W
AU  - Jin WW
AD  - Key Lab of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072.
FAU - Li, X
AU  - Li X
FAU - Li, Z Y
AU  - Li ZY
FAU - Ning, S B
AU  - Ning SB
FAU - Ling, D H
AU  - Ling DH
FAU - Song, Y C
AU  - Song YC
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Shi Yan Sheng Wu Xue Bao
JT  - Shi yan sheng wu xue bao
JID - 0413570
SB  - IM
MH  - Chromosome Mapping
MH  - Gene Dosage
MH  - Gene Expression
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oryza/*genetics
MH  - *Plants, Genetically Modified
EDAT- 2003/01/29 04:00
MHDA- 2003/05/07 05:00
CRDT- 2003/01/29 04:00
PHST- 2003/01/29 04:00 [pubmed]
PHST- 2003/05/07 05:00 [medline]
PHST- 2003/01/29 04:00 [entrez]
PST - ppublish
SO  - Shi Yan Sheng Wu Xue Bao. 2001 Sep;34(3):163-8.