PMID- 1254932
OWN - NLM
STAT- MEDLINE
DCOM- 19760525
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 24
IP  - 1
DP  - 1976 Jan
TI  - Quantitation of lymphocyte response to antigen by flow cytofluorometry.
PG  - 378-82
AB  - The response of human peripheral blood lymphocytes to antigenic stimulation has 
      been studied in vitro using flow cytofluorometry and an acridine orange (AO) 
      staining technique for cellular deoxyribonucleic acid and ribonucleic acid (RNA). 
      Antigen-stimulated "pyroninophillic" immunoblasts, identified by an increase in 
      cellular content of RNA (red fluorescence with AO), were quantitated in 
      triplicate cultures incubated up to 7 days with and without bacterial antigen. 
      These results were similar to 14C-thymidine incorporation into identical cultures 
      incubated in parallel. Cytofluorometric analysis showed a peak in percentage of 
      immunoblasts after 6 days in culture, while maximum thymidine incorporation was 
      seen on day 7. Cells from patients with depressed immune response secondary to 
      cancer showed lower than normal antigen response by cytofluorometry. Kinetic 
      studies revealed both a lower percentage of immunoblasts when compared to normal 
      and a lower average per cell RNA content of the stimulated cells. AO 
      cytofluorometry is suggested as a convenient method of simultaneously assessing 
      lymphocyte proliferative and nonproliferative response to antigen.
FAU - Braunstein, J D
AU  - Braunstein JD
FAU - Good, R A
AU  - Good RA
FAU - Hansen, J A
AU  - Hansen JA
FAU - Sharpless, T K
AU  - Sharpless TK
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Antigens, Fungal)
RN  - 9007-49-2 (DNA)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - *Antigens, Fungal
MH  - Autoanalysis
MH  - Candida
MH  - DNA/biosynthesis
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Lymphocytes/cytology/*immunology/metabolism
MH  - Microscopy, Fluorescence/methods
MH  - Thymidine/metabolism
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1177/24.1.1254932 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1976 Jan;24(1):378-82. doi: 10.1177/24.1.1254932.