PMID- 1254934
OWN - NLM
STAT- MEDLINE
DCOM- 19760525
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 24
IP  - 1
DP  - 1976 Jan
TI  - Cytofluorometric studies on conformation of nucleic acids in situ. I. Restriction 
      of acridine orange binding by chromatin proteins.
PG  - 40-8
AB  - Binding of the fluorochrome acridine orange (AO) to nucleic acids in situ is 
      studied by automated cytofluorometry in two differentiating cell systems: Friend 
      virus-transformed murine erythroleukemia induced to differentiate by dimethyl 
      sulfoxide, and phytohemagglutinin-stimulated human lymphocytes. The specificity 
      of the stain for deoxyribonucleic acid is discussed on the basis of data obtained 
      by cell treatment with nucleases. Evidence is presented that in the case of 
      Friend leukemia cells, but not phytohemagglutinin-stimulated lymphocytes, a 
      significant change in the number of AO-intercalating sites in DNA occurrs during 
      differentiation. These results suggest that changes in nuclear chromatin 
      occurring during cell differentiation may be correlated, in some but not all 
      systems, with changes in accessibility of DNA in situ to intercalating dyes. The 
      role of divalent cations, especially Mg2+, in the conformation of nuclear 
      chromatin and in modulation of the accessibility of nucleic acids to AO is 
      discussed. The method provides a tool for the study of nucleic acid-protein 
      interaction in situ, and in some cell systems it may be applicable as a marker 
      for recognition of cell transformation, differentiation or neoplasia.
FAU - Traganos, F
AU  - Traganos F
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Sharpless, T
AU  - Sharpless T
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Acridines)
RN  - 0 (DNA, Viral)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Lectins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (RNA, Viral)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Acridines
MH  - Cell Line
MH  - Cell Transformation, Neoplastic
MH  - *DNA, Viral
MH  - Deoxyribonucleases
MH  - Humans
MH  - Indicators and Reagents
MH  - Lectins
MH  - Lymphocyte Activation
MH  - Lymphocytes/analysis
MH  - *Nucleic Acid Conformation
MH  - *RNA, Neoplasm
MH  - *RNA, Viral
MH  - Ribonucleases
MH  - Spectrometry, Fluorescence/methods
MH  - Thymus Gland/analysis
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1177/24.1.1254934 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1976 Jan;24(1):40-8. doi: 10.1177/24.1.1254934.