PMID- 1254936
OWN - NLM
STAT- MEDLINE
DCOM- 19760525
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 24
IP  - 1
DP  - 1976 Jan
TI  - Quantitation of fluorescence fading phenomena for identifying intracellular 
      biopolymers.
PG  - 59-63
AB  - The fading behavior of the 670 nm fluorescence emission band produced by unfixed 
      rat mast cells stained with acridine orange (AO) has been found to be in 
      excellent agreement with the behavior predicted by second order chemical 
      kinetics. The reciprocal of fluorescence intensity plotted against time yields a 
      straight line. When due account is taken of dye/cell ratio and the intensity of 
      fluorescence-exciting radiation, Io (measured with the standard phosphor 
      particle), the slope of this straight line is a constant, k'', which is 
      independent of dye/cell ratio and Io. k'' differs from the second order 
      photochemical rate constant by a constant factor. The fading of a given 
      AO-biopolymer complex is described by a particular value of k''. Two values of 
      k'' have been found for rat mast cell granules, indicating the presence of two 
      different AO-biopolymer complexes. Fading of fluorescence may serve to identify 
      particular intracellular biopolymers in individual cells even when present in a 
      heterogeneous population.
FAU - West, S S
AU  - West SS
FAU - Golden, J F
AU  - Golden JF
FAU - Menter, J M
AU  - Menter JM
FAU - Love, L D
AU  - Love LD
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Acridines)
RN  - 0 (Proteins)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acridines
MH  - Animals
MH  - Cytoplasm/analysis
MH  - DNA/*analysis
MH  - Kinetics
MH  - Mast Cells/analysis
MH  - Proteins/*analysis
MH  - RNA/*analysis
MH  - Rats
MH  - Spectrometry, Fluorescence/methods
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1177/24.1.1254936 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1976 Jan;24(1):59-63. doi: 10.1177/24.1.1254936.