PMID- 12550772
OWN - NLM
STAT- MEDLINE
DCOM- 20030224
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 139
IP  - 2
DP  - 2002 Dec
TI  - Application of multicolor fluorescence in situ hybridization analysis for 
      detection of cross-contamination and in vitro progression in commonly used murine 
      tumor cell lines.
PG  - 126-32
AB  - Murine tumor models are potent tools for cancer studies, most of which make use 
      of a limited number of murine tumor cell lines that are exchanged by many 
      research groups around the world. Although cross-contamination and in vitro 
      karyotypic progression are well-known risks with respect to the identity of tumor 
      cell lines, these parameters are rarely evaluated. Notably, routine karyotyping 
      of murine cell lines is laborious and technically demanding because mouse 
      chromosomes are morphologically similar. We therefore used a 21-color 
      fluorescence in situ hybridization (FISH) approach (COBRA) for screening two 
      groups of frequently used murine tumor cell lines, each of which shares known 
      immunologic determinants. Multicolor analysis revealed that the sharing of 
      immunologic determinants among three murine lymphoma cell lines (EL-4, MBL-2, and 
      RBL-5) is directly related to their common origin. In several of the cell lines, 
      the chromosomal derivatives had rearranged further, suggesting that the 
      cross-contamination events were not recent. In contrast, karyotypic analysis of 
      three murine colon cancer cell lines (C26, CC36, and C51) showed that these 
      constituted independent tumor clones despite the sharing of immunologic 
      determinants. Our data point out that cross-contamination and in vitro evolution 
      of murine tumor cell lines are a common phenomenon, and that multicolor FISH 
      analysis is an efficient tool for verifying the origin and tracking the evolution 
      of murine cell lines.
FAU - Wiegant, Joop
AU  - Wiegant J
AD  - Laboratory for Cytochemistry and Cytometry, Department Molecular Cell Biology, 
      Leiden University Medical Center, 2333 AL, Leiden, The Netherlands.
FAU - van Hall, Thorbald
AU  - van Hall T
FAU - van der Burg, Marja
AU  - van der Burg M
FAU - Colombo, Mario
AU  - Colombo M
FAU - Tanke, Hans J
AU  - Tanke HJ
FAU - Offringa, Rienk
AU  - Offringa R
FAU - Rosenberg, Carla
AU  - Rosenberg C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adenocarcinoma/genetics/pathology
MH  - Animals
MH  - Antigens, Neoplasm/genetics
MH  - Cell Culture Techniques/*methods
MH  - Clone Cells/chemistry
MH  - Colonic Neoplasms/genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Lymphoma, T-Cell/genetics/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Neoplasm Proteins/genetics
MH  - Thymoma/genetics/pathology
MH  - Thymus Neoplasms/genetics/pathology
MH  - Tumor Cells, Cultured/*chemistry/immunology
EDAT- 2003/01/29 04:00
MHDA- 2003/02/25 04:00
CRDT- 2003/01/29 04:00
PHST- 2003/01/29 04:00 [pubmed]
PHST- 2003/02/25 04:00 [medline]
PHST- 2003/01/29 04:00 [entrez]
AID - S0165460802006234 [pii]
AID - 10.1016/s0165-4608(02)00623-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Dec;139(2):126-32. doi: 
      10.1016/s0165-4608(02)00623-4.